NASC

NASC ID: N538787

Name: SALK_038787

ABRC stock number: SALK_038787

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_038787.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G56370.1 (at chr 3 pos 20900265 (W/20900206-20900265) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 55-79 bps onto the T-DNA. Pools P 10, R 8, C 3 N 9. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO394830.

SALKseq_038787.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G61830 and AT1G61840.1 (at chr 1 pos 22843281 (C/22843281-22843338) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 10, R 8, C 3 N 9. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO391473.

SALKseq_038787.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G16490.1=P355 and AT4G16500.1=UTR3 (at chr 4 pos 9296676 (W/9296640-9296676) on the TAIR10). The sequences' 1-37 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 10, R 8, C 3 N 9. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO398166.

Donation Date: 2002-01-01

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_038787

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N662161 (SALK_038787C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G61830 AT1G61840 AT4G16490 AT4G16500 At3g56370

EMBL accession:

ED583772

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At3g56370

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.