NASC

NASC ID: N540788

Name: SALK_040788

ABRC stock number: SALK_040788

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_040788.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G11240.1 (at chr 3 pos 3519788 (W/3519728-3519788) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 15, R 15, C 14 N 16. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO394942.

SALKseq_040788.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G39700.1 and AT5G39710.1 (at chr 5 pos 15895838 (C/15895838-15895897) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 15, R 15, C 14 N 16. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO398277.

SALKseq_040788.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G28170.1 and AT3G28180.1=P355 (at chr 3 pos 10504328 (W/10504261-10504328) on the TAIR10). The sequences' 1-68 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 15, R 15, C 14 N 16. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO391583.

Donation Date: 2002-01-01

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_040788

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_040788C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G28170 AT3G28180 AT5G39700 AT5G39710 At3g11240

EMBL accession:

ED586139

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At3g11240

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.