NASC

NASC ID: N561813

Name: SALK_061813

ABRC stock number: SALK_061813

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

April 2002 TAIR gene annotation: F6A14.11 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At1g18790.

Associated Polymorphisms:

SALKseq_061813.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G18790.1 and AT1G18800.1=UTR3 (at chr 1 pos 6480117 (W/6480068-6480117) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 47-77 bps onto the T-DNA. Pools P 35, R 40, C 36 N 31. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO365843.

SALKseq_061813.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G76510.2=P355 and AT1G76520.1=P355 (at chr 1 pos 28714115 (C/28714115-28714164) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 54-78 bps onto the T-DNA. Pools P 35, R 40, C 36 N 31. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO362493.

SALKseq_061813.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G52880.2=P355 and AT3G52890.2=P355 (at chr 3 pos 19606007 (W/19605970-19606007) on the TAIR10). The sequences' 1-38 bps mapped on the genome, 51-78 bps onto the T-DNA. Pools P 35, R 40, C 36 N 31. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO369186.

SALKseq_061813.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G21560.1 (at chr 4 pos 11470555 (C/11470555-11470612) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 35, R 40, C 36 N 31. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO372543.

Donation Date: 2002-04-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_061813

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N662683 (SALK_061813C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G18800 AT1G76510 AT1G76520 AT3G52880 AT3G52890 AT4G21560 At1g18790

EMBL accession:

ED592188

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.