NASC

NASC ID: N568598

Name: SALK_068598

ABRC stock number: SALK_068598

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

May TAIR gene annotation Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At1g20460.

Associated Polymorphisms:

SALKseq_068598.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G20460.1 (at chr 1 pos 7092808 (W/7092756-7092808) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 44, R 48, C 41 N 50. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO369595.

SALKseq_068598.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G79570.1 (at chr 1 pos 29939222 (W/29939162-29939222) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 44, R 48, C 41 N 50. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO366252.

SALKseq_068598.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT2G47350.1 (at chr 2 pos 19437190 (W/19437149-19437190) on the TAIR10). The sequences' 1-42 bps mapped on the genome, 51-78 bps onto the T-DNA. Pools P 44, R 48, C 41 N 50. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO372955.

SALKseq_068598.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G16810.1 and AT4G16820.1 (at chr 4 pos 9466586 (W/9466529-9466586) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 59-76 bps onto the T-DNA. Pools P 44, R 48, C 41 N 50. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO376306.

Donation Date: 2002-05-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_068598

Part of : SALK lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G79570 AT2G47350 AT4G16810 AT4G16820 At1g20460

EMBL accession:

BH848612

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.