NASC

NASC ID: N578857

Name: SALK_078857

ABRC stock number: SALK_078857

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

July 2002 TAIR gene annotation - At1g61780. T13M11.14 R300-5' At1g61790 T13M11.15 L300-5'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_078857.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT1G61780.1 (at chr 1 pos 22814100 (W/22814042-22814100) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 64, R 68, C 68 N 63. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO433621.

SALKseq_078857.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G39670.1 (at chr 2 pos 16533993 (C/16533993-16534052) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 64, R 68, C 68 N 63. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO436924.

SALKseq_078857.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G37530.1=UTR3 and AT4G37540.1=UTR3 (at chr 4 pos 17636930 (W/17636853-17636930) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 64, R 68, C 68 N 63. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO417052.

Donation Date: 2002-01-07

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_078857

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N664710 (SALK_078857C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G39670 AT4G37530 AT4G37540 At1g61780

EMBL accession:

BH854183

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.