NASC

NASC ID: N581332

Name: SALK_081332

ABRC stock number: SALK_081332

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At4g29550 T16L4.6 0 Exon T16L4.6 0 Exon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_081332.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G29550.1 (at chr 4 pos 14502270 (C/14502270-14502327) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 67, R 70, C 66 N 66. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO434017.

SALKseq_081332.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G46770.1 (at chr 2 pos 19222731 (W/19222679-19222731) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 54-79 bps onto the T-DNA. Pools P 67, R 70, C 66 N 66. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO430712.

SALKseq_081332.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G43150.1=UTR3 and AT5G43160.1 (at chr 5 pos 17326186 (W/17326120-17326186) on the TAIR10). The sequences' 1-67 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 67, R 70, C 66 N 66. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO437321.

SALKseq_081332.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G11590.1=P355 and AT5G11600.1=UTR3 (at chr 5 pos 3733288 (W/3733230-3733288) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 56-79 bps onto the T-DNA. Pools P 67, R 70, C 66 N 66. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO417448.

SALKseq_081332.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G24170.1 (at chr 5 pos 8190428 (W/8190377-8190428) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 67, R 70, C 66 N 66. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO420765.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_081332

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_081332C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G46770 AT5G11590 AT5G11600 AT5G24170 AT5G43150 AT5G43160 At4g29550

EMBL accession:

BZ352675

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.