NASC Stock Detail Page
NASC ID: N586774
Name: SALK_086774
ABRC stock number: SALK_086774
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. July 2002 TAIR gene annotation - At4g31010. F6I18.80 Exon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_086774.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G31010.1 (at chr 4 pos 15107816 (C/15107816-15107888) on the TAIR10). The sequences' 1-73 bps mapped on the genome, 72-95 bps onto the T-DNA. Pools P 79, R 80, C 74 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO421788. SALKseq_086774.001 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G31010.1 (at chr 4 pos 15107815 (W/15107741-15107815) on the TAIR10). The sequences' 1-75 bps mapped on the genome, 71-89 bps onto the T-DNA. Pools P 79, R 80, C 74 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO425105. SALKseq_086774.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT1G50110.1 (at chr 1 pos 18560239 (W/18560182-18560239) on the TAIR10). The sequences' 2-59 bps mapped on the genome, 75-95 bps onto the T-DNA. Pools P 79, R 80, C 74 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO438345. SALKseq_086774.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G22560 and AT1G22570.1 (at chr 1 pos 7974816 (W/7974781-7974816) on the TAIR10). The sequences' 1-36 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 79, R 80, C 74 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO418472.
Donation Date: 2002-01-07
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_086774
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N663193 (SALK_086774C).
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.