NASC

NASC ID: N626867

Name: SALK_126867

ABRC stock number: SALK_126867

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_126867.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G60730.1 (at chr 3 pos 22446311 (W/22446255-22446311) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 51-79 bps onto the T-DNA. Pools P 57, R 57, C 56 N 55. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO387032.

SALKseq_126867.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G09370.2=UTR3 and AT3G09375 (at chr 3 pos 2882470 (W/2882430-2882470) on the TAIR10). The sequences' 1-41 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 57, R 57, C 56 N 55. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO390378.

SALKseq_126867.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G08520.1=P355 and AT5G08530.1=UTR3 (at chr 5 pos 2758910 (W/2758857-2758910) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 57, R 57, C 56 N 55. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO393728.

Donation Date: 2003-01-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_126867

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_126867C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G09370 AT3G09375 AT5G08520 AT5G08530 At3g60730

EMBL accession:

BZ764765

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.