NASC Stock Detail Page
NASC ID: N6952
Name: G protein-coupled receptor
ABRC stock number: CS6952
Description: Knockout line harboring a T-DNA insertion in intron 2 (at 506 bp) of the GCR1 gene, was found in a PCR screen of a collection of 72,960 T-DNA-inserted Arabidopsis lines (Arabidopsis Knockout Facility, University of Wisconsin, BASTA population) using a GCR1-specific primer and a T-DNA left border-specific primer; homozygous mutant line for At1g 48270 (GCR1 gene).
Donation Date: 2006-10-16
| Donated by: | Pennsylvania State University Sally Assmann University of Missouri John Walker Franklin and Marshall College Pablo Jenik |
Donor Number: gcr1-3
Part of : Homozygous lines
Stock type: individual line
Material type: seed
Comment: recessive
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: T-DNA Insertion
Background: Ws-2 ( N2360)
Segregation status: homozygous
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
|---|---|---|---|
|
|
|
|
Phenotype
Plants exhibit hypersensitivity to abscisic acid (ABA) in assays of root growth, gene regulation, and stomatal response. Root growth of seedlings is markedly more sensitive to inhibition by ABA compared with wild-type. Plants have enhanced drought tolerance and lower rates of water loss (lower transpiration rates). Plants are hypersensitive to ABA and S1P regulation of stomatal responses: guard cells are hypersensitive to both inhibition of opening of preclosed stomata and promotion of closure of preopened stomata by ABA; plants respond to the lipid metabolite, sphingosine-1-phosphate (S1P), in an identical fashion as they do to ABA: guard cells are hypersensitive to S1P-induced inhibition of stomatal opening and S1P-induced promotion of stomatal closure compared with the wild-type.
References
Pandey, S. & Assmann, S.M. 2004. The Arabidopsis putative G protein-coupled receptor GCR1 interacts with the G protein alpha subunit GPA1 and regulates abscisic acid signaling. The Plant Cell 16(6):1616-32.PubMed ID: 15155892.