NASC Stock Detail Page
NASC ID: N72221
Name: OPT3p::LUC line 3-9
ABRC stock number: CS72221
Description: Transgenic line expressing the firefly luciferase reporter gene under the control of the OPT3 promoter. The 3.0 kb sequence upstream from the start codon of OPT3 was cloned into the pENTR/D-TOPO entry vector. The insert was then transferred to a modified pGreenII 0179 plasmid containing the Luciferase coding sequence. Presence of the insert was confirmed by colony PCR and plasmid sequencing. Col-0 plants were transformed by the floral dip method. Hygromycin resistant. Homozygous for the transgene.
Donation Date: 2019-06-14
| Donated by: | University of Missouri Nga Nguyen University of Missouri David G. Mendoza-Cozatl |
Stock type: individual line
Material type: seed
Comment: OPT3p::LUC
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Seed type: Transgenic
Background: Col(Columbia)
Growth requirement: none
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Luciferase expression induced by Fe-deficient conditions or luciferin treatment.
References
Khan MA, Castro-Guerrero NA, McInturf SA, Nguyen NT, Dame AN, Wang J, Bindbeutel RK, Joshi T, Jurisson SS, Nusinow DA, Mendoza-Cozatl DG; 2018-10-01; Changes in iron availability in Arabidopsis are rapidly sensed in the leaf vasculature and impaired sensing leads to opposite transcriptional programs in leaves and roots.; Plant, Cell & Environment; PubMed ID: 29520929