NASC Stock Detail Page
NASC ID: N72243
Name: ss1551
ABRC stock number: CS72243
Description: Transgenic line expressing self-resistance gene astD. A 35-amino acid residue chloroplast localization signal (CLS) derived from the endogenous A. thaliana DHAD gene was fused to the N-terminus of an A. terreus astD gene codon-optimized for A. thaliana, and a 3xFLAG-tag was inserted in between. The construct was cloned into a pEG202 vector modified to express the transgene from the UBQ10 promoter. Col-0 plants were transformed by the floral dip method. BASTA and aspterric acid resistant. Segregating for the transgene. Independent transgenic line 1; original donated stock is from the T2 generation.
Donation Date: 2019-07-17
| Donated by: | University of California, Los Angeles (UCLA) Steve Jacobsen |
Stock type: individual line
Material type: seed
Comment: pUB10:CLS35-AstD
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Seed type: Transgenic
Growth requirement: Positive transgenic plants can be selected with glufosinate and testing for survival in the presence of aspterric acid.
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
|---|---|---|---|
|
|
|
|
References
Yan Y, Liu Q, Zang X, Yuan S, Bat-Erdene U, Nguyen C, Gan J, Zhou J, Jacobsen SE, Tang Y; 2018-07-01; Resistance-gene-directed discovery of a natural-product herbicide with a new mode of action.; Nature; PubMed ID: 29995859