NASC Stock Detail Page
NASC ID: N72272
Name: p35S-HTA6-GFP
ABRC stock number: CS72272
Description: Transgenic line carrying the CaMV35Spro-HTA6::GFP transgene. The in-frame fusion of HTA6 to the N-terminus of GFP, which is downstream of an enhanced CaMV 35S promoter, was generated by inserting the A. thaliana core histone HTA6 coding sequence (450 bp) into pCsGFPB, a modified pCAMBIA1302 vector containing an sGFP expression cassette. Col-0 plants were transformed by the floral dip method. Hygromycin resistant. Homozygous for the transgene.
Donation Date: 2019-08-12
Donated by: | University of Nebraska David Galbraith |
Stock type: individual line
Material type: seed
Comment: CaMV35Spro-HTA6::GFP
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Seed type: Transgenic
Growth requirement: none
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Green fluorescence (GFP-derived) accumulating within the nuclei throughout the plant. Fluorescence is stably inherited over selfed generations and persists in nuclei isolated in vitro from these plants.
References
Zhang C, Gong FC, Lambert GM, Galbraith DW.; 2005-10-04; Cell type-specific characterization of nuclear DNA contents within complex tissues and organs.; PLANT METHODS; PubMed ID: 16270943