NASC Stock Detail Page
NASC ID: N72273
Name: 35S::GFP:EXO70A3
ABRC stock number: CS72273
Description: Transgenic overexpressor line. The 35Sp::GFP:EXO70A3 construct was generated by Multisite Gateway reaction of building blocks -attL4-35Sp-attR1, attR1-GFP-attL2 and attR2-EXO70A3-attL3 and the pB7m34GW backbone (BASTA selection). GFP, 35S promoter and backbone were obtained from Karimi et al. 2007. EXO70A3 CDS was subcloned into pDONOR P2rP3 (Invitrogen) using BP clonase recombination. Col-0 plants were transformed. Homozygous for the transgene.
Donation Date: 2019-08-15
| Donated by: | Charles University in Prague Ivan Kulich |
Stock type: individual line
Material type: seed
Comment: 35S::GFP:EXO70A3
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Seed type: Transgenic
Growth requirement: none
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
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References
Karimi M, Bleys A, Vanderhaeghen R, Hilson P.; 2007-12-01; Building blocks for plant gene assembly.; PLANT PHYSIOLOGY; PubMed ID: 17965171