NASC Stock Detail Page
NASC ID: N72492
Name: 35S::PIF3-A19:GFP
ABRC stock number: CS72492
Description: Transgenic line. 35S::PIF3-A19:GFP in the pif3-3 mutant background. The full length PIF3 sequence without stop codon was amplified via PCR from a plasmid template and cloned into a Gateway entry vector. 19 S and T residues were subsequently mutated via site directed mutagenesis. LR reaction into binary vector B7FWG was performed and pif3-3 plants were transformed via floral dip. BASTA resistant. Homozygous for the transgene.
Donation Date: 2021-10-02
| Donated by: | The Plant Gene Expression Center Peter Quail The Plant Gene Expression Center Jutta Dalton |
Stock type: individual line
Material type: seed
Comment: Marker:BASTA
| Status | Price (£) |
|---|---|
| Currently unavailable | £11.00 |
Germplasm Info
Genus: Arabidopsis Species: thaliana
Seed type: Transgenic
Background: Col (Columbia)
Growth requirement: none
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Shortened hypocotyls after 4d in the dark
References
Ni, W., Xu, S. L., Chalkley, R. J., Pham, T. N., Guan, S., Maltby, D. A., Burlingame, A. L., Wang, Z. Y., Quail, P. H.; 2013-07-11; Multisite light-induced phosphorylation of the transcription factor PIF3 is necessary for both its rapid degradation and concomitant negative feedback modulation of photoreceptor phyB levels in Arabidopsis; THE PLANT CELL; PubMed ID: 23903316