NASC Stock Detail Page
NASC ID: N72563
Name: AGO4::FLAG-AGO4(D742A) T5-19
ABRC stock number: CS72563
Description: Slicing-defective AGO4 driven by the native AGO4 promoter (pAGO4::FLAG-AGO4-D742A) expressed in the ago4-4/ago6-2/ago9-1 (CS72560) homozygous triple mutant background. Mutagenesis of the catalytic motif of AGO4 was performed by overlapping extension PCR. Hygromycin resistant. Homozygous for the transgene.
Donation Date: 2021-09-07
| Donated by: | Penn State University Michael J Axtell |
Stock type: individual line
Material type: seed
Comment: Marker:BASTA, kanamycin, hygromycin
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Genus: Arabidopsis Species: thaliana
Seed type: Transgenic
Background: Col (Columbia); Ws (Wassilewskija)
Segregation status: homozygous
Growth requirement: none
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Partial complementation of small RNA accumulation
References
Feng Wang, Michael J Axtell; 2017-04-01; AGO4 is specifically required for heterochromatic siRNA accumulation at Pol V-dependent loci in Arabidopsis thaliana; Plant J; PubMed ID: 28002617