NASC Stock Detail Page
NASC ID: N72605
Name: KLR-2
ABRC stock number: CS72605
Description: Independent transgenic line #2 containing the genetically encoded fluorescent biosensor Kinase Localization Reporter (KLR). KLR consists of a bipartite modified nuclear localization signal (bNLS) with two phosphosites, a nuclear localization signal (NLS), the mNeonGreen fluorophore, a T2A "self-cleaving" Thosea asigna virus 2A peptide, the mRuby3 fluorophore, and an NLS derived from simian virus 40 (SV40). This control construct does not contain a kinase docking domain, which is an essential component of a fully functional Kinase Localization Reporter. Primary transformants were identified with an epifluorescence microscope and seed was collected from plants exhibiting strong fluorescence. Transgene genotype unknown.
Donation Date: 2021-08-24
| Donated by: | University of Wisconsin Patrick Krysan |
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available to order at NASC | £11.00 |
Germplasm Info
Genus: Arabidopsis Species: thaliana
Seed type: Transgenic
Background: Col (Columbia)
Growth requirement: screen seedlings for fluorescence
Phenotype
Red and green fluorescence present within nuclei of cells
References
Kati Seitz, Patrick J Krysan; 2020-07-28; Expanding the Toolkit of Fluorescent Biosensors for Studying Mitogen Activated Protein Kinases in Plants; Int J Mol Sci; PubMed ID: 32731410