NASC Stock Detail Page
NASC ID: N72608
Name: KLR-EE-1
ABRC stock number: CS72608
Description: Independent transgenic line #1 containing the genetically encoded fluorescent biosensor Kinase Localization Reporter variant KLR-EE. KLR-EE consists of a bipartite modified nuclear localization signal (bNLS) with two phosphosites, a nuclear localization signal (NLS), the mNeonGreen fluorophore, a T2A "self-cleaving" Thosea asigna virus 2A peptide, the mRuby3 fluorophore, and an NLS derived from simian virus 40 (SV40). The two phosphosites within the bNLS have been mutagenized to phosphomimetic glutamic acids. This control construct does not contain a kinase docking domain. Primary transformants were identified with an epifluorescence microscope and seed was collected from plants exhibiting strong fluorescence. Transgene genotype unknown.
Donation Date: 2021-08-24
| Donated by: | University of Wisconsin Patrick Krysan |
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available to order at NASC | £11.00 |
Germplasm Info
Genus: Arabidopsis Species: thaliana
Seed type: Transgenic
Background: Col (Columbia)
Growth requirement: screen seedlings for fluorescence
Phenotype
Red fluorescence present within the nuclei of cells, green fluorescence excluded from the nuclei of cells
References
Kati Seitz, Patrick J Krysan; 2020-07-28; Expanding the Toolkit of Fluorescent Biosensors for Studying Mitogen Activated Protein Kinases in Plants; Int J Mol Sci; PubMed ID: 32731410