NASC Stock Detail Page
NASC ID: N72610
Name: KLR-MKP1-1
ABRC stock number: CS72610
Description: Independent transgenic line #1 containing the genetically encoded fluorescent biosensor Kinase Localization Reporter KLR-MKP1. KLR-MKP1 consists of an 80 aa section of the Arabidopsis protein MAPK Phosphotase 1 (MKP1), which serves as a kinase docking domain, a bipartite modified nuclear localization signal (bNLS) with two phosphosites, a nuclear localization signal (NLS), the mNeonGreen fluorophore, a T2A "self-cleaving" Thosea asigna virus 2A peptide, the mRuby3 fluorophore, and an NLS derived from simian virus 40 (SV40). Primary transformants were identified with an epifluorescence microscope and seed was collected from plants exhibiting strong fluorescence. Transgene genotype unknown.
Donation Date: 2021-08-24
| Donated by: | University of Wisconsin Patrick Krysan |
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available to order at NASC | £11.00 |
Germplasm Info
Genus: Arabidopsis Species: thaliana
Seed type: Transgenic
Background: Col (Columbia)
Growth requirement: screen seedlings for fluorescence
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Red fluorescence present within the nuclei of cells, green fluorescence present within cells
References
Kati Seitz, Patrick J Krysan; 2020-07-28; Expanding the Toolkit of Fluorescent Biosensors for Studying Mitogen Activated Protein Kinases in Plants; Int J Mol Sci; PubMed ID: 32731410