NASC Stock Detail Page
NASC ID: N72616
Name: KLR-AA-AP2C1-1
ABRC stock number: CS72616
Description: Independent transgenic line #1 containing the genetically encoded fluorescent biosensor Kinase Localization Reporter KLR-AA-AP2C1. KLR-AA-AP2C1 consists of a 35 aa section of the Arabidopsis protein PP2C-type phosphotase AP2C1, which serves as a kinase docking domain, a bipartite modified nuclear localization signal (bNLS) with two phosphosites, a nuclear localization signal (NLS), the mNeonGreen fluorophore, a T2A "self-cleaving" Thosea asigna virus 2A peptide, the mRuby3 fluorophore, and an NLS derived from simian virus 40 (SV40). The two phosphosites within the bNLS have been mutagenized to non-phosphorylable alanines. Primary transformants were identified with an epifluorescence microscope and seed was collected from plants exhibiting strong fluorescence. Transgene genotype unknown.
Donation Date: 2021-08-24
| Donated by: | University of Wisconsin Patrick Krysan |
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Currently unavailable | £11.00 |
Germplasm Info
Genus: Arabidopsis Species: thaliana
Seed type: Transgenic
Background: Col (Columbia)
Growth requirement: screen seedlings for fluorescence
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Red and green fluorescence present within the nuclei of cells
References
Kati Seitz, Patrick J Krysan; 2020-07-28; Expanding the Toolkit of Fluorescent Biosensors for Studying Mitogen Activated Protein Kinases in Plants; Int J Mol Sci; PubMed ID: 32731410