Home | About NASC | Address/Staff
Ask a question | Help

NASC

The European Arabidopsis Stock Centre

NASC Stock Detail Page

NASC ID: N72736

Name: GFP-PGX3

ABRC stock number: CS72736

Description: Transgenic line expressing a PGX3-GFP fusion protein driven by the PGX3 promoter (ProPGX3:PGX3-GFP). A 2072-bp promoter-containing fragment upstream of the PGX3 start codon and the full PGX3 CDS were amplified from Col genomic DNA and Col cDNA, respectively, then ligated by overlap PCR. The final product was cloned into pMDC110 to generate the ProPGX3:PGX3-GFP construct, which was then transformed into Col plants. Hygromycin resistant. Homozygous for the transgene.

Donation Date: 2021-11-17

Donated by:  Pennsylvania State University Avry Mudersbach
Pennsylvania State University Charles Thomas Anderson

Stock type: individual line

Material type: seed

Comment: Marker:hygromycin

Status Price (£)
Currently unavailable £11.00

Germplasm Info

Genus: Arabidopsis     Species: thaliana

Seed type: Transgenic

Background: Col (Columbia)

Growth requirement: none

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:
  • pgx3-1

References

Yue Rui, Chaowen Xiao, Hojae Yi, Baris Kandemir, James Z Wang, Virendra M Puri, Charles T Anderson; 2017-10-01; POLYGALACTURONASE INVOLVED IN EXPANSION3 Functions in Seedling Development, Rosette Growth, and Stomatal Dynamics in Arabidopsis thaliana; Plant Cell; PubMed ID: 28974550