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NASC

The European Arabidopsis Stock Centre

NASC Stock Detail Page

NASC ID: N72743

Name: PLL12-comp

ABRC stock number: CS72743

Description: PLL12 complementation line. The 2 kb PLL12 native promoter and PLL12 coding sequence were ligated and cloned into the pCR8/GW/TOPO entry vector, then LR cloned into vector pMDC110. The plasmid was transformed into pll12-1 (SAIL_1207_A07/CS878465) heterozygous plants and selected using methionine sulfoximine and hygromycin to obtain homozygous lines for both alleles.

Donation Date: 2021-11-17

Donated by:  Pennsylvania State University Avry Mudersbach
Pennsylvania State University Charles Thomas Anderson

Stock type: individual line

Material type: seed

Comment: Marker:BASTA, hygromycin

Status Price (£)
Available £11.00

Germplasm Info

Genus: Arabidopsis     Species: thaliana

Seed type: Transgenic

Background: Col (Columbia)

Segregation status: homozygous

Growth requirement: none

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:
  • pll12-1

References

Yintong Chen, Wenlong Li, Joseph A Turner, Charles T Anderson; 2021-09-24; PECTATE LYASE LIKE12 patterns the guard cell wall to coordinate turgor pressure and wall mechanics for proper stomatal function in Arabidopsis; Plant Cell; PubMed ID: 34109391