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NASC

The European Arabidopsis Stock Centre

NASC Stock Detail Page

NASC ID: N72755

Name: pHAM3::2xYPET-N7mirS in Ler

ABRC stock number: CS72755

Description: Transgenic microRNA-sensitive fluorescence reporter line for HAM3. The 3,816 bp HAM3 promoter was amplified and cloned before 2xYPET-N7mirS; and the 1,755 bp HAM3 3?ÇÖ untranslated sequence was PCR amplified and cloned 3?ÇÖ of the 2xYPEY-N7mirS fragment. The fused DNA fragment was introduced into pMOA34 and Ler plants were transformed. Hygromycin selection. Homozygous for the transgene.

Donation Date: 2021-11-29

Donated by:  California Institute of Technology (Caltech) Elliot Meyerowitz

Stock type: individual line

Material type: seed

Comment: Marker:hygromycin

Status Price (£)
Available £11.00

Germplasm Info

Genus: Arabidopsis     Species: thaliana

Seed type: Transgenic

Background: Ler (Landsberg erecta)

Growth requirement: hygromycin selection

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:

References

Yun Zhou, Xing Liu, Eric M Engstrom, Zachary L Nimchuk, Jose L Pruneda-Paz, Paul T Tarr, An Yan, Steve A Kay, Elliot M Meyerowitz; 2015-01-15; Control of plant stem cell function by conserved interacting transcriptional regulators; Nature; PubMed ID: 25363783