NASC Stock Detail Page
NASC ID: N72761
Name: mddcc
ABRC stock number: CS72761
Description: Quintuple mutant (met1-8, drm1-2, drm2-2, cmt3-11, cmt2-3) generated by CRISPR/Cas9-mediated mutation of MET1 directly in the ddcc quadruple mutant background. Two sgRNAs were designed to target the first exon of the MET1 gene and the CRISPR-Cas9 construct was transformed into the ddcc mutant background. Then, heterozygous met1 mutants (met1/+) without the CRISPR-Cas9 construct were isolated from the mutated T2 populations in the ddcc background. Deposited seeds are the progenies of met1/+ drm1 drm2 cmt3 cmt2 plants and were used to isolate mddcc DNA methylation-free quintuple mutant plants.
Donation Date: 2022-04-04
| Donated by: | Chinese Academy of Sciences Li He |
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Seed type: Transgenic
Mutagen: CRISPR/Cas9
Background: Col-0/Salk_074069
Segregation status: segregating
Growth requirement: none
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
|---|---|---|---|
|
|
|
Phenotype
Quintuple homozygous plants exhibit extreme growth retardation and small size, display a suite of severe developmental defects, and do not undergo floral transition.
References
Li He, Huan Huang, Mariem Bradai, Cheng Zhao, Yin You, Jun Ma, Lun Zhao, Rosa Lozano-Durá, Jian-Kang Zhu; 2022-04-14; DNA methylation-free Arabidopsis reveals crucial roles of DNA methylation in regulating gene expression and development; Nat Commun.; PubMed ID: 35288562