NASC Stock Detail Page
NASC ID: N73270
Name: mbd5 mbd6 CRISPR1
ABRC stock number: CS73270
Description: mbd5 mbd6 double mutant generated by CRISPR/Cas9. A CRISPR guide for the MBD5 gene was cloned in the AtU6-26-sgRNA cassette and the PCR product was cloned into the pYAO::hSpCas9 destination plasmid. The final vector was transformed in mbd6-1 mutant plants (derived from SALK_043927) by floral dipping. The CRISPR/Cas9 transgene has been segregated out.
Donation Date: 2022-10-21
| Donated by: | University of California, Los Angeles (UCLA) Steve Jacobsen |
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Seed type: Transgenic
Background: Col (Columbia)
Segregation status: homozygous
Marker used: kanamycin
Growth requirement: none