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NASC ID: N821715

Name: SAIL_514_D11

ABRC stock number: CS821715

Description: T-DNA insertion line, generated by vacuum infiltration of a Columbia (Col) plant with Agrobacterium tumefaciens vector pDAP101, a modified approach of Thermal Asymmetric Interlaced-Polymerase Chain Reaction (TAIL-PCR) was used to amplify DNA fragments flanking the T-DNA left border from this transformed line; BASTA was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually as part of the Syngenta Arabidopsis Insertion Library (SAIL) collection.

Associated Polymorphisms:

SAILseq_514_D11.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G08140.1 (at chr 1 pos 2554505 (W/2554412-2554505) on the TAIR10). The sequences' 1-94 bps mapped on the genome. Pools P 25, R 27, C 24 N 22. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO336683.

SAILseq_514_D11.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G19820.2-P355 and AT3G19830.1-UTR3 (at chr 3 pos 6884934 (C/6884934-6884980) on the TAIR10). The sequences' 1-47 bps mapped on the genome, 52-81 bps onto the T-DNA. Pools P 25, R 27, C 24 N 22. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO328087.

SAIL_514_D11

Donation Date: 2004-06-01

Donated by:  Syngenta AG Alberto Martinez

Donor Number: SAIL_514_D11

Part of : SAIL lines from Syngenta

Stock type: individual line

Material type: seed

Status Price (£)
Available to order at NASC £10.00

Germplasm Info

Background: Col-0( N60000)

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:
  • ATCHX6
EMBL accession:

References

McElver, J. et al. 2001. Insertional mutagenesis of genes required for seed development in Arabidopsis thaliana. Genetics 159(4):1751-63.PubMed ID: 11779812.