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NASC Stock Detail Page

NASC ID: N9602

Name: myb12 KO

ABRC stock number: CS9602

Description: Alleles to stock:myb12-1f. The isolation of insertion mutants from the En/Spm mutagenized AMAZE2 population (Wisman et al., 1998) was described previously (Meissner et al., 1999). HPLC analysis of the progeny of the homozygous myb12::En-1 line that did display wild-type-like PCR fragments led to the recovery of the homozygous myb12-1f line, which showed reduced levels of flavonols in the absence of an En-1 element at the MYB12 locus. This line was backcrossed five times to wild type (accession Col-0) to give rise to an En-free homozygous myb12-1f mutant. The myb12-1f allele was checked by sequencing. PCR analysis confirmed the absence of intact En-elements in the genome of myb12-1f plants.

Donation Date: 2006-12-01

Donated by:  Bielefeld University Bernd Weisshaar

Donor Number: S_RS_509

Stock type: individual line

Material type: seed

Comment: gene product:MYB12, PRODUCTION OF FLAVONOL GLYCOSIDE1 (PFG1)

Status Price (£)
Available £10.00

Germplasm Info

Seed type: En/Spm Sequence tagged

Mutagen: transposon insertion

Background: Col-0 (N60000)

Segregation status: homozygous

Pedigree: Backcrossed five times to wild type (Col-0).

Segregation: no

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:
  • myb12
  • myb12
EMBL accession:

Phenotype

Results from the myb12-1f allele indicate that an activation domain might be present in the C-terminus. myb12 knock-out plants do not show any obvious phenotype under greenhouse conditions. Young myb12-ko seedlings contain reduced amounts of flavonoids (quercetin and kaempferol). They did not show any significant difference in anthocyanin content. Expression of CHS and FLS shows a clear correlation to MYB12 expression levels. CHI and F3H expression levels show no differences in the knock-out. Even in the absence of functional MYB12, flavonol biosynthesis is not completely absent, suggesting functional redundancy.

References

Rosso, M.G. et al. 2003. An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for flanking sequence tag-based reverse genetics. Plant molecular biology 53(1-2):247-59.PubMed ID: 14756321.

Mehrtens, F. et al. 2005. The Arabidopsis transcription factor MYB12 is a flavonol-specific regulator of phenylpropanoid biosynthesis. Plant Physiology 138(2):1083-96.PubMed ID: 15923334.