Home | About NASC | Address/Staff
Ask a question | Help

NASC

From January 1st, 2021, seed orders from EU customers will require a phytosanitary certificate. Please click for further information

NASC Stock Detail Page

NASC ID: N9603

Name: MYB12 OX

ABRC stock number: CS9603

Description: Alleles to stock: MYB12-OX, 35S-MYB12. The construct used for plant transformation was based on the binary vector pGPTV-TATA, a derivative of pGPTV (Becker et al., 1992) containing theTATA-GUS cassette of pBT10 (Sprenger-Haussels and Weisshaar, 2000). Plants were transformed by infiltration using Agrobacterium tumefaciens strain GV3101 (Koncz and Schell, 1986). For overexpression, the 35S-MYB12 cassette of p35S-MYB12 (see below) was amplified with theprimers HK122 (5#-TTCATTAAAGCTTCGGATCCATCGATG-3#; introduction of a HinDIII restriction site) and HK123 (5#-CATGATGAGCTCGTTGACAGAAGCCAAGCG-3#; introduction of a SacI restriction site). The 35S-MYB12cassette was introduced into pGPTV-TATA by replacing the TATA-GUS sequences and transformed into Arabidopsis accession Col-0. Transformants were selected on media containing kanamycin.

Donation Date: 2006-12-01

Donated by:  Bielefeld University Bernd Weisshaar

Donor Number: S_RS_606

Stock type: individual line

Material type: seed

Comment: gene product:MYB12, PRODUCTION OF FLAVONOL GLYCOSIDE1 (PFG1)

Status Price (£)
Available £10.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-0 (N60000)

Segregation status: homozygous

Pedigree: Backcrossed five times to wild type (Col-0).

Segregation: no

Transformation method: The construct used for plant transformation was based on the binary vector pGPTV-TATA, a derivative of pGPTV (Becker et al., 1992) containing theTATA-GUS cassette of pBT10 (Sprenger-Haussels and Weisshaar, 2000). Plants were transformed by infiltration using Agrobacterium tumefaciens strain GV3101 (Koncz and Schell, 1986). For overexpression, the 35S-MYB12 cassette of p35S-MYB12 (see below) was amplified with theprimers HK122 (5#-TTCATTAAAGCTTCGGATCCATCGATG-3#; introduction of a HinDIII restriction site) and HK123 (5#-CATGATGAGCTCGTTGACAGAAGCCAAGCG-3#; introduction of a SacI restriction site). The 35S-MYB12cassette was introduced into pGPTV-TATA by replacing the TATA-GUS sequences and transformed into Arabidopsis accession Col-0. Transformants were selected on media containing kanamycin.

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:
  • MYB12
  • myb12

Phenotype

Seedlings as well as leaves of MYB12-OX plants displayed an increased flavonoid content. They did not show any significant difference in anthocyanin content. Expression of CHS and FLS shows a clear correlation to MYB12 expression levels. CHI and F3H show increased transcript levels in the MYB12-OX line.

References

Mehrtens, F. et al. 2005. The Arabidopsis transcription factor MYB12 is a flavonol-specific regulator of phenylpropanoid biosynthesis. Plant Physiology 138(2):1083-96.PubMed ID: 15923334.