How were the SINS sequences derived:

Sequencing primers and direction:

Superpool	Template generation	Sequencing primer	Recommended primer for PCR
01_x x_x x.txt 02_x x_x x.txt 03_x x_x x.txt 04_x x_x x.txt 05_x x_x x_1.txt 01_x x_x x b.txt 02_x x_x x b.txt 03_x x_x x b.txt 04_x x_x x b.txt 05_x x_x x_5.txt	Adaptor PCR - Mse I Adaptor PCR - Mse I Adaptor PCR - Mse I Adaptor PCR - Mse I IPCR - BstY I Adaptor PCR - Bfa I Adaptor PCR - Bfa I Adaptor PCR - Bfa I Adaptor PCR - Bfa I IPCR - BstY I	spm3 spm3 spm3 spm3 dspm1 spm3 spm3 spm3 spm3 dspm5	dspm1 dspm1 dspm1 dspm1 dspm1 dspm1 dspm1 dspm1 dspm1 dspm5

**Note 1: All sequences for superpool 05 were generated from individual lines - all others were generated from pools.
**Note 2: In the SINS database, all dspm sequence has been removed from the flanking sequence.

Other recommended primers and positions in dSPM

Preferred primers - sequences
dspm1 CTT ATT TCA GTA AGA GTG TGG GGT TTT GG
dSpm8130: GAG CGT CGG TCC CCA CAC TTC TAT AC
dSpm8166: GTC CAT TTT AGA GTG ACG GCT AAG AGT G
dSpm8: GTT TTG GCC GAC ACT CCT TAC C
dspm5: CGG GAT CCG ACA CTC TTT AAT TAA CTG ACA CTC
dSpm11: GGT GCA GCA AAA CCC ACA CTT TTA CTT C