David Bouchez
I. N. R. A.
Laboratoire de Biologie Cellulaire
78026 Versailles cedex FRANCE
tel (33) (1) 30 83 33 94
fax (33) (1) 30 83 30 99
e-mail : bouchez@versailles.inra.fr
(Reconstructed) DNA sequence of T-DNA pGKB5 (7599 b.p.)
Note :The 8 kb replication origin from pRiA4 is inserted in the NotI site (position 83). This is a BamHI fragment with NotI linkers on both ends.
Bluescript polylinker : 1-104
Right Border : 105-622
24 bp sequence : 574-596
GUS coding : 638-2446
NOS terminator : 2505-2766
OCS terminator : 2767-3489
KanR (nptII) : 3490-4479
NOS promoter : 4480-4766
35S promoter : 4767-5924
BastaR (bar) : 5925-6513
g7 terminator : 6514-6768
Left Border : 6790-7525
24 bp sequence : 6963-6986
Bluescript polylinker : 7526-7599
GGAAACAGCTATGACCATGATTACGCCAAGCTCGGAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGCTCCACCGCGGTGGCGGCCGCTCTAGAGGATCCCCCCACAGACAGCTCCGTAGCCCTCGTTCTCCTTGGAGTTCTTCGGGAAATGGATCTTTCGATTCCCGATGATGTCTCTCTTATCTGCTTTGACGACGCCGACTGGACATCCGCTATAACGCCGCCATTGACCGTGATTTCGCAACCTGTCAGGGATCTCGCGACGGCTGCCACAGAAGACCTGATCGCCCGCTTAAAGGGCGAGACTTCAGCCCCACCCAAGGAAACTCTTCTCCCGGCGGTTCTCATAGAGCGCGGTTCCGTAAGCGGTTCTTCGCAAGGTCGGGGTTGCATACCGAACTCGCGAAACGTCGGCGACTGAGCTCCCGAGGCGCGTTGACAAGATGCCACGAAGGGAATGGAAGACAGCCGATATTGCAATTGTCTTCGTGGACTGCTTTCGGGACGTAAGGCGCAAGCCATCATCACCGCCGTCCTAAACAAACATACCTCCACACAAATTTATCTACCTGACCACAAGATATATCCTGTCACACGATTTATTAAACGCTGCACTTGGGGTGGTCAGTCCCTTATGTTACGTCCTGTAGAAACCCCAACCCGTGAAATCAAAAAACTCGACGGCCTGTGGGCATTCAGTCTGGATCGCGAAAACTGTGGAATTGATCAGCGTTGGTGGGAAAGCGCGTTACAAGAAAGCCGGGCAATTGCTGTGCCAGGCAGTTTTAACGATCAGTTCGCCGATGCAGATATTCGTAATTATGCGGGCAACGTCTGGTATCAGCGCGAAGTCTTTATACCGAAAGGTTGGGCAGGCCAGCGTATCGTGCTGCGTTTCGATGCGGTCACTCATTACGGCAAAGTGTGGGTCAATAATCAGGAAGTGATGGAGCATCAGGGCGGCTATACGCCATTTGAAGCCGATGTCACGCCGTATGTTATTGCCGGGAAAAGTGTACGTATCACCGTTTGTGTGAACAACGAACTGAACTGGCAGACTATCCCGCCGGGAATGGTGATTACCGACGAAAACGGCAAGAAAAAGCAGTCTTACTTCCATGATTTCTTTAACTATGCCGGAATCCATCGCAGCGTAATGCTCTACACCACGCCGAACACCTGGGTGGACGATATCACCGTGGTGACGCATGTCGCGCAAGACTGTAACCACGCGTCTGTTGACTGGCAGGTGGTGGCCAATGGTGATGTCAGCGTTGAACTGCGTGATGCGGATCAACAGGTGGTTGCAACTGGACAAGGCACTAGCGGGACTTTGCAAGTGGTGAATCCGCACCTCTGGCAACCGGGTGAAGGTTATCTCTATGAACTGTGCGTCACAGCCAAAAGCCAGACAGAGTGTGATATCTACCCGCTTCGCGTCGGCATCCGGTCAGTGGCAGTGAAGGGCCAACAGTTCCTGATTAACCACAAACCGTTCTACTTTACTGGCTTTGGTCGTCATGAAGATGCGGACTTACGTGGCAAAGGATTCGATAACGTGCTGATGGTGCACGACCACGCATTAATGGACTGGATTGGGGCCAACTCCTACCGTACCTCGCATTACCCTTACGCTGAAGAGATGCTCGACTGGGCAGATGAACATGGCATCGTGGTGATTGATGAAACTGCTGCTGTCGGCTTTAACCTCTCTTTAGGCATTGGTTTCGAAGCGGGCAACAAGCCGAAAGAACTGTACAGCGAAGAGGCAGTCAACGGGGAAACTCAGCAAGCGCACTTACAGGCGATTAAAGAGCTGATAGCGCGTGACAAAAACCACCCAAGCGTGGTGATGTGGAGTATTGCCAACGAACCGGATACCCGTCCGCAAGTGCACGGGAATATTTCGCCACTGGCGGAAGCAACGCGTAAACTCGACCCGACGCGTCCGATCACCTGCGTCAATGTAATGTTCTGCGACGCTCACACCGATACCATCAGCGATCTCTTTGATGTGCTGTGCCTGAACCGTTATTACGGATGGTATGTCCAAAGCGGCGATTTGGAAACGGCAGAGAAGGTACTGGAAAAAGAACTTCTGGCCTGGCAGGAGAAACTGCATCAGCCGATTATCATCACCGAATACGGCGTGGATACGTTAGCCGGGCTGCACTCAATGTACACCGACATGTGGAGTGAAGAGTATCAGTGTGCATGGCTGGATATGTATCACCGCGTCTTTGATCGCGTCAGCGCCGTCGTCGGTGAACAGGTATGGAATTTCGCCGATTTTGCGACCTCGCAAGGCATATTGCGCGTTGGCGGTAACAAGAAAGGGATCTTCACTCGCGACCGCAAACCGAAGTCGGCGGCTTTTCTGCTGCAAAAACGCTGGACTGGCATGAACTTCGGTGAAAAACCGCAGCAGGGAGGCAAACAATGAATCAACAACTCTCCTGGCGCACCATCGTCGGCTACAGCCTCGGTGGGGAATTGAGCTCGATCGTTCAAACATTTGGCAATAAAGTTTCTTAAGATTGAATCCTGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATGTAATGCATGACGTTATTTATGAGATGGGTTTTTATGATTAGAGTCCCGCAATTATACATTTAATACGCGATAGAAAACAAAATATAGCGCGCAAACTAGGATAAATTATCGCGCGCGGTGTCATCTATGTTACTAGATCGAATTCGATCGAGGGGATCGAGCCCCTGCTGAGCCTCGACATGTTGTCGCAAAATTCGCCCTGGACCCGCCCAACGATTTGTCGTCACTGTCAAGGTTTGACCTGCACTTCATTTGGGGCCCACATACACCAAAAAAATGCTGCATAATTCTCGGGGCAGCAAGTCGGTTACCCGGCCGCCGTGCTGGACCGGGTTGAATGGTGCCCGTAACTTTCGGTAGAGCGGACGGCCAATACTCAACTTCAAGGAATCTCACCCATGCGCGCCGGCGGGGAACCGGAGTTCCCTTCAGTGAACGTTATTAGTTCGCCGCTCGGTGTGTCGTAGATACTAGCCCCTGGGGCCTTTTGAAATTTGAATAAGATTTATGTAATCAGTCTTTTAGGTTTGACCGGTTCTGCCGCTTTTTTTAAAATTGGATTTGTAATAATAAAACGCAATTGTTTGTTATTGTGGCGCTCTATCATAGATGTCGCTATAAACCTATTCAGCACAATATATTGTTTTCATTTTAATATTGTACATATAAGTAGTAGGGTACAATCAGTAAATTGAACGGAGAATATTATTCATAAAAATACGATAGTAACGGGTGATATATTCATTAGAATGAACCGAAACCGGCGGTAAGGATCTGAGCTACACATGCTCAGGTTTTTTACAACGTGCACAACAGAATTGAAAGCAAATATCATGCGATCATAGGCGTCTCGCATATCTCATTAAAGCAGGGGGTGGGCGAAGAACTCCAGCATGAGATCCCCGCGCTGGAGGATCATCCAGCCGGCGTCCCGGAAAACGATTCCGAAGCCCAACCTTTCATAGAAGGCGGCGGTGGAATCGAAATCTCGTGATGGCAGGTTGGGCGTCGCTTGGTCGGTCATTTCGAACCCCAGAGTCCCGCTCAGAAGAACTCGTCAAGAAGGCGATAGAAGGCGATGCGCTGCGAATCGGGAGCGGCGATACCGTAAAGCACGAGGAAGCGGTCAGCCCATTCGCCGCCAAGCTCTTCAGCAATATCACGGGTAGCCAACGCTATGTCCTGATAGCGGTCCGCCACACCCAGCCGGCCACAGTCGATGAATCCAGAAAAGCGGCCATTTTCCACCATGATATTCGGCAAGCAGGCATCGCCATGGGTCACGACGAGATCCTCGCCGTCGGGCATGCGCGCCTTGAGCCTGGCGAACAGTTCGGCTGGCGCGAGCCCCTGATGCTCTTCGTCCAGATCATCCTGATCGACAAGACCGGCTTCCATCCGAGTACGTGCTCGCTCGATGCGATGTTTCGCTTGGTGGTCGAATGGGCAGGTAGCCGGATCAAGCGTATGCAGCCGCCGCATTGCATCAGCCATGATGGATACTTTCTCGGCAGGAGCAAGGTGAGATGACAGGAGATCCTGCCCCGGCACTTCGCCCAATAGCAGCCAGTCCCTTCCCGCTTCAGTGACAACGTCGAGCACAGCTGCGCAAGGAACGCCCGTCGTGGCCAGCCACGATAGCCGCGCTGCCTCGTCCTGCAGTTCATTCAGGGCACCGGACAGGTCGGTCTTGACAAAAAGAACCGGGCGCCCCTGCGCTGACAGCCGGAACACGGCGGCATCAGAGCAGCCGATTGTCTGTTGTGCCCAGTCATAGCCGAATAGCCTCTCCACCCAAGCGGCCGGAGAACCTGCGTGCAATCCATCTTGTTCAATCCACATGATCATGGGCCGGATCTTTGATTGAGAGTGAATATGAGACTCTAATTGGATACCGAGGGGAATTTATGGAACGTCAGTGGAGCATTTTTGACAAGAAATATTTGCTAGCTGATAGTGACCTTAGGCGACTTTTGAACGCGCAATAATGGTTTCTGACGTATGTGCTTAGCTCATTAAACTCCAGAAACCCGCGGCTGAGTGGCTCCTTCAATCGTTGCGGTTCTGTCAGTTCCAAACGTAAAACGGCTTGTCCCGCGTCATCGGCGGGGGTCATAACGTGACTCCCTTAATTCTCCGCTCATGATCCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACATTATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGAATTGACGGATCTCCTTTGCCCCGGAGATCACCATGGACGACTTTCTCTATCTCTACGATCTAGGAAGAAAGTTCGACGGAGAAGGTGACGATACCATGTTCACCACCGATAATGAGAAGATTAGCCTCTTCAATTTCAGAAAGAATGCTGACCCACAGATGGTTAGAGAGGCCTACGCGGCAGGTCTCATCAAGACGATCTACCCGAGTAATAATCTCCAGGAGATCAAATACCTTCCCAAGAAGGTTAAAGATGCAGTCAAAAGATTCAGGACTAACTGCATCAAGAACACAGAGAAAGATATATTTCTCAAGATCAGAAGTACTATTCCAGTATGGACGATTCAAGGCTTGCTTCATAAACCAAGGCAAGTAATAGAGATTGGAGTCTCTAAGAAAGTAGTTCCTACTGAATCAAAGGCCATGGAGTCAAAAATTCAGATCGAGGATCTAACAGAACTCGCCGTGAAGACTGGCGAACAGTTCATACAGAGTCTTTTACGACTCAATGACAAGAAGAAAATCTTCGTCAACATGGTGGAGCACGACACTCTCGTCTACTCCAAGAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCTATTGAGACTTTTCAACAAAGGGTAATATCGGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTCATCAAAAGGACAGTAGAAAAGGAAGGTGGCACCTACAAATGCCATCATTGCGATAAAGGAAAGGCTATCGTTCAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACACGCTGAAATCACCAGTCTCTCTCTACAAATCGGATCCATGAGCCCAGAACGACGCCCGGCCGACATCCGCCGTGCCACCGAGGCGGACATGCCGGCGGTCTGCACCATCGTCAACCACTACATCGAGACAAGCACGGTCAACTTCCGTACCGAGCCGCAGGAACCGCAGGAGTGGACGGACGACCTCGTCCGTCTGCGGGAGCGCTATCCCTGGCTCGTCGCCGAGGTGGACGGCGAGGTCGCCGGCATCGCCTACGCGGGCCCCTGGAAGGCACGCAACGCCTACGACTGGACGGCCGAGTCGACCGTGTACGTCTCCCCCCGCCACCAGCGGACGGGACTGGGCTCCACGCTCTACACCCACCTGCTGAAGTCCCTGGAGGCACAGGGCTTCAAGAGCGTGGTCGCTGTCATCGGGCTGCCCAACGACCCGAGCGTGCGCATGCACGAGGCGCTCGGATATGCCCCCCGCGGCATGCTGCGGGCGGCCGGCTTCAAGCACGGGAACTGGCATGACGTGGGTTTCTGGCAGCTGGACTTCAGCCTGCCGGTACCGCCCCGTCCGGTCCTGCCCGTCACCGAGATCTGATCTCACGCGTCTAGGATCCGATGGATCCCCCGATGAGCTAAGCTAGCTATATCATCAATTTATGTATTACACATAATATCGCACTCAGTCTTTCATCTACGGCAATGTACCAGCTGATATAATCAGTTATTGAAATATTTCTGAATTTAAACTTGCATCAATAAATTTATGTTTTTGCTTGGACTATAATACCTGACTTGTTATTTTATCAATAAATATTTAAACTATATTTCTTTCAAGATGGGAATTAACATCTACAAATTGCCTTTTCTTATCGACCATGTACATCAAGCTTATCGATACCGTCGGCTATTGGTAATAGGACACTGGGATTCGTCTTGGACAACTTTCCTTCTCATCTAAGCGTAGACAACCCTCAACTGGAAACGGGCCGGACTCCAGGGCGTGTGCCAGGTGCCCACGGAATAGTTTTGGCCAGACCCTTGAAAATCCGATTCAGTACAATCGATTGCCCTCATTTTTACGTTGGCATATATCCTGCCAAACAGCCAACAACGCGCGTGCGGTGAATAGGAAAGCGTTTGAGTTGCTTGCTCATATCGTGACGGTTGACAGCACAGGTTGACCGCTTGATGATTCGTACGAGCCGCCAAACATTGGCTGTCGTAATGATATACCATGTCAGAACAGCAATCCGATGGGGCGGAAAGCATTATCTTAATGCACACGGAAATGGCGCGTCGGTGGGTGGAATACACCGACATAGAGGCCGTAAGTTCTGCATGGTCATCGTCGGAAAGGTGGCAGCAGGCGCACGGCTGTGGCCTCTTGCTCTTTCAGCGTGAAATGCGTGTTGAAAGAATAATCGAAGAGAGCGTCCGCTCGACACCTTCAATTATGCCGATTTGATCGATGAACTGATCGAGCTCTGAAATCGAAGGGGCTTCGATAATCGCAATCAAATCAAAAGTGCCACTCACAGAATGAAGAGCGATAACGGCCGTGACCTTCCCAAGGGAGGCCGTCACCTGTGAAAGCGCCTTCGTAATGGTGATCAGAATATGGGCTCGAACCAAGCTCGAGACCTCGAGGGGGGGCCCGGTACCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTAC