Introduction To Classical Development Techniques

Classical genetic methods based on crosses and selection schemes have made enormous contributions towards stress-related crop improvement however, the problem with traditional plant breeding is that it is time consuming and laborious; it is difficult to modify single traits; and it relies on existing genetic variability.

Modern Transformation Techniques

Two main types of method for plant transformation exist, the use of Agrobacterium as a biological vector for foreign gene transfer, and direct gene transfer techniques, in which DNA is introduced into cells by the use of physical, electrical or chemical means.

-Agrobacterium

-Direct Gene Transfer (DGT)