This condition was carried out because it was noticed that when the pots were removed from the -20ºC freezer after 4 hours (condition 6) the temperature of the top soil only reached -8ºC. It was prudent therefore, to attempt a more revised condition for stratification. It was found however that the temperature of the soil only reached -15ºC in this condition . It took 4 days for the seeds to start germinating after they had been removed from the freezer where they were conditioned at -20 ºC for 8 hours (overnight). Again the N933 strain started germinating the most rapidly with 12 seeds germinating by the 3 rd day. Again the N20 started germinating quicker (initially) when compared to the N1601 ecotype, however by the 4 th day the N1601 strain had germinated more seed than the N20 ecotype. True leaves started developing by the 8 th day in the N933 strain, and by the end of the experiment all of the 15 germinated seeds of N933 had developed into true leaves.
Cond8 vs Cond1 |
Ttest |
N20-A |
0.72 |
N20-B |
0.17 |
N1601-A |
0.16 |
N1601-B |
0.38 |
N933-A |
0.57 |
N933-B |
0.69 |
It appears as though none of the ecotypes statistically benefited from the freezer treatment at -20 ºC. Again the N1601 strain has a relatively low p value of 0.16 when compared to the N20-A p value of 0.72 and the N933-A p value of 0.57. However yet again it failed to prove a significant improvement in the rate of germination. However the rate of development into true leaves was reduced in the N20 strain giving a value of 0.17. Again however this is not statistically significant.
Abstract,Introduction,Germination,Dormancy,Temperature,Light,Ecotypes
Ecotype-Storage,Soil preperation,Seed Sowing,Germination-Conditions,Measurement of Results
Cond1,Cond1rep,Cond2,Cond3,Cond4,Cond5,Cond6,Cond6rep,Cond7,Cond8