Three different ecotypes were used for this experiment. The first is the NW20 strain (Landsberg erecta (Ler-0)). Its' phenotype exhibits ‘ blunt fruits, short petioles, usually upright stems, compact inflorescence; carries erecta gene; blunt siliques, bunched flowers; common background line (http://arabidopsis.info). George Redei originally received a stock of seed from Laibach with the name Landsberg, on which he performed some X-ray mutagenesis experiments. During these experiments it became clear that the original Landsberg sample was not homogeneous but a mixture of slightly different types.
The Landsberg erecta line was selected from the population of Landsberg seed that had been irradiated. It was called Landsberg erecta by Will Feenstra and was the background line for all the mutants that have subsequently been produced by Feenstra, Van der Veen and Koornneef at Wageningen University . Therefore the true breeding line Landsberg erecta is not the same as the Landsberg ecotype in the AIS Collection (N1298). For this reason the Landsberg erecta line should be considered a separate background Ler-0 (NW20) instead of La-0. La-0 referring to the AIS Landsberg (N1298 http://arabidopsis.info)) .
The second is the N1601 or Ws-2 parental line for Feldmann T-DNA tagged lines. All of the Feldmann transformed T-DNA lines are in the Wassilewskija background. Note that the Ws accession utilised for these experiments was maintained for a number of generations in U.S. laboratories, and a stock of this line has been made available through the stock centres Ws-2 ( http://arabidopsis.info).
The third is N933 or Colombia (Col-4) strain. There are several Columbia accessions, 0 through 7, all genetically very similar. In many cases when parental accessions to the progenitor of a population are unambiguous a new stock name is allocated in the form Col-n. The original Columbia ecotype was selected by Redei from the no irradiated Laibach Landsberg population (isolate 5-13) as it was a particularly fertile and vigorous plant that responded well to changes in photoperiod. The line I am using is one that was used as the parental for the recombinant inbred population of Caroline Dean ( http://arabidopsis.info) .
Abstract,Introduction,Germination,Dormancy,Temperature,Light,Ecotypes
Ecotype-Storage,Soil preperation,Seed Sowing,Germination-Conditions,Measurement of Results
Cond1,Cond1rep,Cond2,Cond3,Cond4,Cond5,Cond6,Cond6rep,Cond7,Cond8
Discussion,Future-experiments,References