MOGEN promoter-trapping lines donated to NASC

P.C. Sijmons
Einsteinweg 97,
2333 CB Leiden,
The Netherlands
phone +31-71-258282
fax +31-71-221471

In September 1994, almost 800 transgenic Arabidopsis lines from MOGEN (Leiden, The Netherlands) were deposited at the Nottingham Arabidopsis Stock Centre. The lines carry at least one T-DNA insert from pMOG553 which has a promoterless GUS gene close to the right border sequence and a hygromycin resistance marker. The lines can be used for research purposes and will be available initially from the Nottingham Arabidopsis Stock Centre after signing a specific release form. The origin of the pMOG533/Arabidopsis lines must be duly acknowledged when used in publications.

These lines will be released by NASC as soon as possible

To facilitate molecular analysis of the MOGEN lines by other research groups, I have included all relevant data that is presently available (construct details, transformation protocol used, etc.)


The deposited lines come from a collection of over 1250 independent transformants that were originally made to study gene regulation in nematode-feeding structures. Its use is first described in:

Goddijn, O.J.M. Lindsey, K. Vanderlee, F.M. Klap, J.C. & Sijmons, P.C. Differential gene expression in nematode-induced feeding structures of transgenic plants harbouring promoter gusA fusion constructs. Plant J. (1993) 4:863-873.


The Arabidopsis ecotype C24 (Lehle Seeds, Arizona, USA) was used for the root transformation protocol described by Valvekens et al (PNAS, 85:5536-5540, 1988) with minor alterations. Seeds were germinated in liquid germination medium (appendix 1) for 1 week (100rpm rotary shaker, 23C, 1000 lux (16h L / 8h D). Roots harvested from the germinated seedlings were directly used for co cultivation with Agrobacterium tumefaciens (strain MOG101, C58 chromosomal background, binary vector pMOG553). Hygromycin shoots were allowed to root and transferred to soil.

Plant Growth & Seed Production

All plant growth was done in growth chambers (16h L / 8h D) at 21C and ca. 40-60% RH in Aracon tubes (Beta Developments, Gent, Belgium, phone +32-9-251-7375) to prevent cross-pollination. The primary transformants were grown to maturity and harvested individually (S1 seeds). Seeds were stored at 10% RH, 8C until further analysis (see below). For bulking, seeds were plated without surface sterilization on a selective germination medium (GM + 10mg/ml hygromycin) and kept at 4C for 4 days and were then transferred to RT with moderate light conditions for ca. 10 days. Usually 8-10 hygromycin resistant seedlings were transferred to potting soil and grown to maturity in Aracon tubes. The seeds harvested from the 8-10 plants (S2 seeds) were pooled. It is from these S2 batches that aliquots were deposited in Nottingham. The aliquots thus contain both heterozygote and homozygote seeds.

GUS analysis and available data

Most of the presently available data was recorded through analysis of seedlings from S1 seeds, except for those lines that yielded only very few S1 seeds; these were bulked prior to GUS analysis. When released all the information generated through the analysis of these lines by MOGEN will be made public. This information includes:

Non-available lines

A number of lines are not available through Nottingham. These lines were either hygromycin-sensitive escapes or identified in a screen by us or by our collaborators and are used for more detailed research. I expect that they will become available after publication or when we are notified that they can be released into the public domain. The following categories (or selections thereof) are not yet available:

Important Notes

Please send research data that is of general interest (segregation analysis, number of T-DNA inserts etc.) as much as possible to the Nottingham Stock Centre. It will be used to keep the database up-to-date.

In case you want some more detailed information, you can contact:

Dr. Stephan Ohl				Dr. Peter C. Sijmons
MOGEN international NV			Current Address: ATO-DLO
Einsteinweg 97 17
2333 CB  LEIDEN				6700 AA  Wageningen
The Netherlands				The Netherlands
fax +31-71-221471			fax +31-8370-12260