(1)ABRC, The Ohio State University, 1735 Neil Avenue, 309 Botany & Zoology Bldg. Columbus, OH 43210, USA.
AIMS Web server URL= http://genesys.cps.msu.edu:3333
(2)NASC, Dept Life Science, Nottingham University, Nottingham, NG7 2RD, UK.
NASC WWW server URL= http://nasc.nott.ac.uk/
The stock centers have been working hard in the past few months to add new stocks to the collections. Many new seed stocks and clones have been received recently. In this connection a seed donation campaign, conducted jointly by ABRC and NASC, is in progress. Hence, this update will be devoted mainly to the topic of new seed donations.
The stocks received to date (combined for ABRC and NASC) are listed below.
Description Donor aba-1, ga1-3 R. Wilson adh R. Wilson adh, py-201 R. Wilson adh1 R. Dolferus adh1 R. Dolferus an R. Wilson axr4-1 L. Hobbie axr4-2 L. Hobbie axr4-2, aux1-7 L. Hobbie axr4-2, axr1-3 L. Hobbie axr4-2, axr1-12 L. Hobbie clv3-2 D. Smyth ctr1-1 J. Ecker cyr1 J. Deikman dim1-1 T. Takahashi ein3-1 J. Ecker ein4 J. Ecker ein5-1 J. Ecker ein6 J. Ecker ein7 J. Ecker eir1-1 J. Ecker eto2 J. Ecker eto3 J. Ecker fad2-1 C. Somerville fad2-1 J. Browse fad3# (transgenic) C. Somerville fad3-2 C. Somerville fad7 fad8 C. Somerville fad7-2 J. Browse fey-1 J. Medford fus3-3 P. McCourt fus 4-1 S.Misera fus5-1 S.Misera fus11-1 S.Misera fus12-1 S.Misera gai, an R. Wilson gai, py-201 R. Wilson gar1, gai, an R. Wilson gar2, gai, an R. Wilson gar3, gai, an R. Wilson gar4, gai, an R. Wilson gar5, gai, an R. Wilson gar6, gai, an R. Wilson gar7, gai, an R. Wilson gar8, gai, an R. Wilson gar9, gai, an R. Wilson gar10, gai, an R. Wilson gar11, gai, an R. Wilson gar12, gai, an R. Wilson gar13, gai, an R. Wilson gar14, gai, an R. Wilson gar15, gai, an R. Wilson gar16, gai, an R. Wilson gar17, gai, an R. Wilson gld C. Somerville ilr1-1 B. Bartel jar1-1 P. Staswick lec1-1 D. Meinke lug-1 Z. Liu phyB overex.(transgenic) D. Wagner pid-1 D. Smyth pid-2 D. Smyth pid-3 D. Smyth pin-1 D. Smyth pfl M. Van Lijsebettens qrt1-1 D. Preuss qrt2-1 D. Preuss spy-5 R. Wilson stm, gl1 C. Somerville stp1 T. Baskin ts4 C. Somerville Ac/Ds lines: Ac1 (Stock# CS8043) V. Sundaresan Ac2 (Stock# CS8044) V. Sundaresan Ac5 (Stock# CS8045) V. Sundaresan DsE1 (Stock# CS8046) V. Sundaresan DsE2 (Stock# CS8047) V. Sundaresan DsE3 (Stock# CS8048) V. Sundaresan DsG1 (Stock# CS8049) V. Sundaresan Ac Lines: SPT :: Ac(Bg) -> - 6 C.Dean SPT :: Ac(Bg) -> - 7 C.Dean SPT :: Ac -> - 1 C.Dean SPT :: Ac -> -10 C.Dean SPT :: Ac -> -15 C.Dean SPT :: Ac Nae1 -> - 2 C.Dean SPT :: Ac Nae1 -> - 3 C.Dean SPT :: Ac Nae1 <- - 3 C.Dean SPT :: Ac Nae1 <- -10 C.Dean Buckhorn Pass ecotype A. Murphy Berkeley ecotype A. Murphy Santa Clara ecotype A. Murphy Limeport ecotype A. Murphy Tol-0 ecotype S. Leisner Tol-1 ecotype S. Leisner Tol-2 ecotype S. Leisner Tol-3 ecotype S. Leisner Tol-4 ecotype S. Leisner Tol-5 ecotype S. Leisner Tol-6 ecotype S. Leisner Tol-7 ecotype S. Leisner Tol-8 ecotype S. Leisner Tol-9 ecotype S. Leisner Arabidopsis himalaica H. Tsukaya 100 RI Lines Col-5 X Nd-1 E. Holub, J. Beynon, I. CruteNOTE that these stocks are not currently available - with one exception. The ABRC has accelerated the release process with the Ac/Ds stocks donated by Dr. Sundaresan, and these may be ordered from ABRC immediately. Many of the remainder will be available very soon. We will post notices to the Arabidopsis newsgroup when this occurs.
|We greatly appreciate the generosity of the donors in sharing their stocks. We hope that the cooperative spirit evidenced by these donations will continue.|
Other Ac lines have been donated by Caroline Dean. These lines were produced by introducing the maize element Ac into Landsberg erecta by Agrobacterium - mediated transformation. The Ac element is inserted into the 5'untranslated leader of a streptomycin phosphotransferase (SPT) gene (Dean et al  Plant Journal 2, 69-81). SPT::Ac -> lines contain the wild type Ac in the same orientation as the SPT gene. In SPT::Ac (BG)-> lines the Ac element has a Bgl 11 restriction site inserted downstream of the transposase polyadenylation site. Both these lines are homozygous for the T-DNA. The Nae1Ac lines contain an Ac derivative, lacking 537bp of the CpG-rich 5' untranslated leader of the transposase. These lines show a higher excision frequency and are described in Lawson et al  Mgg 245, 608-615. Excision of the Ac element from the SPT gene results in resistance to streptomycin. Each Nae1 Ac line is selfed progeny from a highly variegated individual and should result in germinal excision events in the next generation.
The ABRC has a large new collection of DNA stocks, including specifically large insert libraries and filters made from these. Consult previous issues of Weeds World for details of these stocks.
The Web pages of NASC (NASC) and AIMS) can be consulted for additional details of all available Arabidopsis stocks.