Figure 3: Representative DNA gel blot analysis of DNA from plants from Line
1926.
DNA was isolated from individual plants. The genotype of these plants was
determined by scoring in the next generation for those plants that produced
seed. Lanes are labelled as follows : MM - homozygous male fertile plants,
Mm - heterozygous plants, and mm - homozygous male sterile plants. The DNA
was cleaved with EcoRI, resolved on an agarose gel, transferred onto nylon,
and hybridised to a radiolabelled probe derived from the right border of
the T-DNA. Sizes (in kilobases) of the DNA fragments are shown on the left.
DNA representing 126 individual F2 plants was analysed in this manner. All
three T-DNA hybridising bands segregated with the ms mutation in these plants.
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