Table 1. RFLP mapping using the Ler x Sn(5)-1 recombinant lines.

Molecular markers
Enzyme used to generate RFLP
Ratio
Sn(5)-1
/Totala
Marker used for recombinant selection
Determined
map position
(cM)
Integrated map positionb
(cM)
2632
EcoRV
4/34
lulu
19.0
27.4
pCITf16
Sty1, Dra1
5/21
lulu
20.5
26.9
4111
HindIII
52/72
lulu
26.4
28.0
PI cosmid
PvuII
56/72
lulu
27.1
28.4
4560
Sty1
61/72
lulu
27.9
27.9
EG7G2 -Lc
HindIII
50/72
lulu
unlinked
-
TSL
-
no RFLP detected
lulu
-
28.7
(0.8 cM from 4560d)
lambda9B3e
Pst1
72/72
77/77
lulu
ttgttg
29.8
-
 
NIT4 (CAPS)f
MboII
72/72
77/77
lulu
ttgttg
29.8
-
 
pCIT718
BglII
65/77
ttgttg
30.7
24.9
21503
Bsc1
59/77
ttgttg
31.1
-
KG10
Bsc1, EcoRV
51/76
ttgttg
31.7
35.5
CDPK.9
-
22/71
ttgttg
33.7
-
 

a = Ratio of number of recombinants showing Sn(5)-1 genotype at the marker locus/Total number of recombinants analysed

b = Hauge et al. (1993)

c = Left end-probe derived from EG7G2 YAC, this YAC was later shown to be chimeric

d = Map position based on distance from 4560 with RI lines (Lister and Dean, 1993)

e = lambda clone identified by screening a genomic library (Voytas et al., 1990) with right end-probe of the YAC CIC9B3

f = Bartel and Fink (1994)