Table 1. RFLP mapping using the Ler x Sn(5)-1 recombinant
lines.
Molecular markers
|
Enzyme used to generate RFLP
|
Ratio
Sn(5)-1
/Totala
|
Marker used for recombinant selection
|
Determined
map position
(cM)
|
Integrated map positionb
(cM)
|
2632
|
EcoRV
|
4/34
|
lulu
|
19.0
|
27.4
|
pCITf16
|
Sty1, Dra1
|
5/21
|
lulu
|
20.5
|
26.9
|
4111
|
HindIII
|
52/72
|
lulu
|
26.4
|
28.0
|
PI cosmid
|
PvuII
|
56/72
|
lulu
|
27.1
|
28.4
|
4560
|
Sty1
|
61/72
|
lulu
|
27.9
|
27.9
|
EG7G2 -Lc
|
HindIII
|
50/72
|
lulu
|
unlinked
|
-
|
TSL
|
-
|
no RFLP detected
|
lulu
|
-
|
28.7
(0.8 cM from 4560d)
|
lambda9B3e
|
Pst1
|
72/72
77/77
|
lulu
ttgttg
|
29.8
|
-
|
NIT4 (CAPS)f
|
MboII
|
72/72
77/77
|
lulu
ttgttg
|
29.8
|
-
|
pCIT718
|
BglII
|
65/77
|
ttgttg
|
30.7
|
24.9
|
21503
|
Bsc1
|
59/77
|
ttgttg
|
31.1
|
-
|
KG10
|
Bsc1, EcoRV
|
51/76
|
ttgttg
|
31.7
|
35.5
|
CDPK.9
|
-
|
22/71
|
ttgttg
|
33.7
|
-
|
a = Ratio of number of recombinants
showing Sn(5)-1 genotype at the marker locus/Total number of recombinants
analysed
b = Hauge et al.
(1993)
c = Left end-probe derived from
EG7G2 YAC, this YAC was later shown to be chimeric
d = Map position based on distance
from 4560 with RI lines (Lister and Dean, 1993)
e = lambda clone identified
by screening a genomic library (Voytas et al., 1990) with right
end-probe of the YAC CIC9B3
f = Bartel and Fink (1994)