Table 2. RFLP mapping using the Ler x Ws recombinant lines. 

Molecular markers
Enzyme used to generate RFLP
Ratio
Ws/Totala
Determined map position
(cM)
Integrated map positionb
(cM)
4111
EcoR1
34/34
< 29.8c
28.0
4560
EcoR1
34/34
< 29.8c
27.9
lambda 9B3d
-
no polymorphism
-
-
NIT4 (CAPS)e
-
no polymorphismf
-
-
pCIT718
BglII
22/34
31.8
24.9
21503
EcoR1
17/34
32.7
-
KG10
EcoR1
17/34
32.7
35.5
4556
EcoR1
1/34
35.3
30.7
6843
EcoR1
0/34
>35.5
32.5
 

a = Ratio of number of recombinants showing Ws genotype at the marker locus/Total number of recombinants analysed

b = Hauge et al. (1993)

c = map position cannot be resolved using this recombinant population

d = lambda clone identified by screening genomic library (Voytas et al., 1990) with right end probe of the YAC CIC9B3

e = Bartel and Fink (1994)

no polymorphismf = Ase1 was reported as generating a polymorphism between Ler and Ws (Bartel and Fink, 1994), however using our material no polymorphism was detected.