NASC

N70500

Name: JYB151.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pATR9::ER-GFP:NOS construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: epidermis and cortex from late maturation zone but fussy (2/5, 5DAG, 1% sucrose); no expression (5; 5DAG; 4.5% sucrose).

N70501

Name: JYB155.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pATR9::ER-GFP:NOS construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: maturation zone on epidermis and cortex (4/6, 2/5)-6 DFG; strong cortex expression in 4.5% sucrose (4/5 6DAG).

N70502

Name: JYB155.2

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pATR9::ER-GFP:NOS construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: maturation zone on epidermis (5 out of 6)-6 DFG, cortex expression and likely in endodermis is stronger than in epidermis (2/5 6DAG); endodermis expression is deemer in 4.5% sucrose (3/5 6DAG).

N70503

Name: JYB155.3

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pATR9::ER-GFP:NOS construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: maturation zone on epidermis and cortex (4 out of 5)-6 DFG.

N70504

Name: JYB158.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pS2 (AT3G61850)::cS2:GFP(no Gly linker):NOS construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: nuclear localized GFP expression in the pericycle and other vascular tissues above the elongation zone (4/6; 6 DFG).

N70505

Name: JYB160.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pS2 (AT3G61850)::cS2:GFP(no Gly linker):NOS construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: nuclear localized GFP expression possibly starts from xylem, later in pericycle and phloem (4/6; 7 and 8DFG).

N70506

Name: JYB232.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pLRC1-4(AT1G08930)::ER-GFP:NOS construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: strong expression in epidermis (atrichoblast) and cortex from meristematic and early elongation zone, and lateral root cap and columella (fussy and non specific), weak expression in endodermis and possibly quiescent center (5/6; 7DFG); but no expression in stele; at 4.5% sucrose, lateral root cap and epidermis in meristematic zone and elongation zone. From maturation zone atrichoblast and cortex, later only in cortex (5/5; 7DAG); at 1%, there is early starting of expression in cortex in meristematic zone, overall expression is weaker than 4.5% (4/5; 7DAG).

N70507

Name: JYB233.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pLRC1-4(AT1G08930)::ER-GFP:NOS construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: patchy expression in epidermis from early elongation zone and lateral root cap and columella two bottom layers (5/5; 6DFG); at 4.5% sucrose GFP expression in epidermis starting from late meristematic zone and patchy expression in lateral root cap and cortex expression starts in late maturation zone (3/5; 7DAG); at 1% expression is weaker than 4.5% (3/3; 7DAG).

N70508

Name: JYB234.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pLRC1-4(AT1G08930)::ER-GFP:NOS construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: patchy expression in epidermis from early elongation zone (5/5; 6DFG); there is strong expression in cortex from maturation zone (3/5; 6DAG; 4.5% sucrose), same in 1% sucrose.

N70509

Name: JYB234.2

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pLRC1-4(AT1G08930)::ER-GFP:NOS construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: patchy expression in epidermis (atrichoblast) from early elongation zone and lateral root cap and columella two bottom layers; weak expression in endodermis and possibly quiescent center (4/6; 5DFG).

N70510

Name: JYB240.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pLRC3-5(AT1G30650)::ER-GFP:NOS construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: GFP in two or three bottom layers of columella and lateral root cap (3/5; 5DFG).

N70511

Name: JYB240.2

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pLRC3-5(AT1G30650)::ER-GFP:NOS construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: GFP in two or three bottom layers of columella and lateral root cap (2/5; 5DFG).

N70512

Name: JYB290.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pE29(At4g05170)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: endodermis maturation zone and above (5/5).

N70513

Name: JYB291.2

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pE29(At4g05170)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: endodermis maturation zone and above (3/5).

N70514

Name: JYB293.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pE29(At4g05170)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: endodermis maturation zone and above (3/5).

N70515

Name: JYB293.2

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pE29(At4g05170)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: endodermis maturation zone and above (2/5).

N70516

Name: JYB295.3

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pE29(At4g05170)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: endodermis maturation zone and above (3/6).

N70517

Name: JYB296.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pATR8(AT3G48100)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: weak on lateral root cap and possibly in stele procambium. From promeristem and fade quickly (2/5, 7DAG, 1% sucrose); lateral root cap and bottom two layers of columella and stele procambium, expression is stronger (3/5; 7DAG; 4.5% SUC).

N70518

Name: JYB297.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pATR8(AT3G48100)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: lateral root cap and columella from second layer (very strong) and stele procambium, meristematic and elongation zone (5/6; 3/5 6DAG); sporadic epididermis expression (4/5-6DAG)-not found in secondary observation; GFP in columella/lateral root cap/procambium (3/4; 5DAG); at 4.5% sucrose, stele procambium expression stops in elongation zone and columella expression is weaker (3/5, 6DAG).

N70519

Name: JYB297.2

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pATR8(AT3G48100)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: weaker expression than line CS70518 (5/6; 5DAG).

N70520

Name: JYB297.3

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pATR8(AT3G48100)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: strong procambium expression (5/6).

N70521

Name: JYB328.4

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pE31(At4g28890)::ER-GFP:NOS#1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: endodermis mature, after long root hair (2/4; 4% sucrose; 6DAG - 4/5 ;1% sucrose; 7DAG); endodermis after maturation zone (3/4; 5DAG).

N70522

Name: JYB336.3

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pE31(At4g28890)::ER-GFP:NOS#1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: endodermis after maturation zone (5/6; 5DAG).

N70523

Name: JYB349.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pE32(At4g32710)::ER-GFP:NOS #2 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: endodermis mature, after root hair emergence (2/6; 6DAG); no GFP (5DAG).

N70524

Name: JYB352.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pE32(At4g32710)::ER-GFP:NOS #2 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: no expression (6/6; 6DAG - 5/5; 4% sucrose; 5DAG); fussy GFP around endodermis of maturation zone.

N70525

Name: JYB357.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pE36(AT5G06840)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: endodermis mature, strong (4/5; 7DAG).

N70526

Name: JYB357.2

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pE36(AT5G06840)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: endodermis mature, weak (3/5; 7DAG).

N70527

Name: JYB359.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pE36(AT5G06840)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: endodermis mature starting before root hair (2/6 7DAG).

N70528

Name: JYB373.2

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pS9(AT1G52150)::cS9:GFP(-G):NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: nuclear localized GFP expression in the middle of the stele (not in pericycle) of the meristematic zone (4/6; 6DAG).

N70529

Name: JYB374.2

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pS9(AT1G52150)::cS9:GFP(-G):NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: nuclear localized GFP expression in the middle of the stele (not in pericycle) of the meristematic zone (1/7; 6DAG).

N70530

Name: JYB375.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pS9(AT1G52150)::cS9:GFP(-G):NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on plates and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: nuclear localized GFP expression in the stele (not in pericycle) of the meristematic zone (2/7; 5DAG).

N70531

Name: JYB394.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pE18(At1g69490)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: quiescent center, pericycle around xylem, a possibly endodermis in meristematic zone (2/6; 6DAG).

N70532

Name: JYB395.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pE18(At1g69490)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: quiescent center and last two columella tiers and CE initial, fainter in most of meristematic zone (5/5).

N70533

Name: JYB395.2

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pE18(At1g69490)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: quiescent center and CE initial, fainter in most of meristematic zone (4/4; 6DAG).

N70534

Name: JYB397.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pE18(At1g69490)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: quiescent center, cortex endo stele in meristematic zone, possibly columella (3/5; 6DAG).

N70535

Name: JYB406.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pS17(AT2G22850)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: GFP in two pericycle in phloem pole starting from early elongation zone (2/3; 4DAG; 3/4-6DAG).

N70536

Name: JYB407.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pS17(AT2G22850)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: GFP in phloem starting from early elongation zone(4/6; 4DAG).

N70537

Name: JYB408.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pS17(AT2G22850)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: GFP in two pericycle in phloem pole starting from early elongation zone (5/6)-4DAG; 3/3-6DAG.

N70538

Name: JYB410.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pS17(AT2G22850)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: GFP in two pericycle in phloem pole starting from early elongation zone (7/9; 6DAG).

N70539

Name: JYB416.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pS19(AT4G37940)::cS19:GFP(-G):NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: very weak nuclear localized GFP in stele of meristimatic zone (4/6; 6DAG).

N70540

Name: JYB421.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pS19(AT4G37940)::cS19:GFP(-G):NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: nuclear localized GFP in stele of meristematic zone, sometimes in cortex in elongation zone (6/7; 6DAG).

N70541

Name: JYB424.2

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pS29(AT2G37590)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: GFP expression in only two cell files of phloem precursor (1/6; 5DAG); no GFP (3; 6DAG; 4.5% sucrose); none (1% sucrose; 4DAG).

N70542

Name: JYB426.2

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pS29(AT2G37590)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: GFP expression in phloem precursor in the meristematic zone (3/5; 5DAG); number of cell files with GFP is only 2-4 (3/; 6DAG; 4.5% sucrose).

N70543

Name: JYB431.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pS27(AT1G68130)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: usual weak expression in the stele (2/5; 6DAG); weak expression in the percycle and cortex in elongation zone (2/6; 5DAG), stele expression seems background; not clear in 4.5% sucrose (5; 6DAG).

N70544

Name: JYB432.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pS27(AT1G68130)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: GFP in pericycle and cortex, in elongation zone and stele in maturation zone (5/6; 5DAG); no specific expression except for the stele in maturation zone and looks like background (5; 7DAG; 4.5% sucrose).

N70545

Name: JYB440.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pE39(AT5G23920)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: endodermis weak mature (1/6; 6DAG).

N70546

Name: JYB440.2

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pE39(AT5G23920)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: endodermis mature, beginning of long root hair (4/5; 6DAG).

N70547

Name: JYB442.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pE39(AT5G23920)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: endodermis mature, nice and strong (5/5; 6DAG).

N70548

Name: JYB442.2

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pE39(AT5G23920)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: endodermis mature, nice and strong (4/5; 6DAG).

N70549

Name: JYB444.1

Price: £11.00

Donor Duke University Philip Benfey Cornell University Ji-Young Lee

Stock type: individual line

Material type: seed

Description Transgenic GFP-fusion expression line harboring the pE39(AT5G23920)::ER-GFP:NOS #1 construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.

Phenotype Transcription factor (TF) expressed in a tissue-enriched manner in roots: endodermis mature, nice and strong (6/6; 6DAG).