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Search result for '29 '. Viewing records 201 to 250 of 972 hits.



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N101211 Name: AT_329 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101212 Name: AT_3388 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101213 Name: AT_3508 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101214 Name: AT_11 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101215 Name: AT_3257 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101216 Name: AT_73 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101217 Name: AT_3004 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101218 Name: AT_478 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101219 Name: AT_3094 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101220 Name: AT_3163 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101221 Name: AT_3237 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101222 Name: AT_68 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101223 Name: AT_3155 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101224 Name: AT_63 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101225 Name: AT_314 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101226 Name: AT_300 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101227 Name: AT_64 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101228 Name: AT_3529 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101229 Name: AT_3366 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101230 Name: AT_3411 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101231 Name: AT_321 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101232 Name: AT_3414 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101233 Name: AT_3409 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101234 Name: AT_3486 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101235 Name: AT_3234 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101236 Name: AT_181 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101237 Name: AT_3240 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101238 Name: AT_3238 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101239 Name: AT_99 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101240 Name: AT_95 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101241 Name: AT_3375 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101242 Name: AT_120 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101243 Name: AT_3171 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101244 Name: AT_116 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101245 Name: AT_35 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101246 Name: AT_3026 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101247 Name: AT_113 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101248 Name: AT_3412 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101249 Name: AT_136 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101250 Name: AT_3528 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101251 Name: AT_3524 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101252 Name: AT_31 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101253 Name: AT_3145 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101254 Name: AT_3188 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101255 Name: AT_118 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101256 Name: AT_121 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101257 Name: AT_3167 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101258 Name: AT_138 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101259 Name: AT_3024 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N101260 Name: AT_3149 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
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