NASC

N3874

Name: sucrose insensitive

Price: £11.00

Donor Rice University Sue Gibson

Locus SIS

Stock type: individual line

Material type: seed

Description Form

Phenotype small and compact rosette, short hypocotyl, short inflorescence, short roots with abundant root hairs; sugar resistant and osmo-tolerant phenotype: seeds germinate and develop into seedlings with expanded cotyledons and true leaves on media containing inhibitory concentrations of mannose (e.g. 1.7 - 3 mM), glucose (e.g. 0.27 - 0.33 M), sucrose (e.g. 0.27 - 0.33 M), sorbitol (e.g. 0.4 - 0.5 M), NaCl (e.g. 0.2 M) or betaine (e.g. 0.45M); resistant to the negative effects of 0.12 mM paclobutrazol (inhibitor of gibberellin biosynthesis on seed germination); not backcrossed to wt; allelic to ctr1-12. homozygous

N3875

Name: resistant to NPA

Price: £11.00

Donor Brown University Alison DeLong

Locus RCN1

Stock type: individual line

Material type: seed

Description Biochemical, Form

Phenotype decreased root and hypocotyl elongation in dark grown seedlings; apical hypocotyl hook defect (a partial hook forms); irregular waving and reversed slant in root waving assay; increased sensitivity to phosphatase inhibitors cantharidin and okadaic acid; encodes an A regulatory subunit of protein phosphatase 2A; homozygous for kanamycin resistance; backcrossed 3 times to Ws. Homozygous

N6360

Name: cytokinin-independent

Price: £11.00

Donor University of Georgia Melissa Pischke University of Wisconsin, Madison Michael Sussman

Locus CKI1

Stock type: individual line

Material type: seed

Description lethal when homozygous, not transmitted through the female gametophyte; % transmission following self-fertilization of heterozygous plants is 50%, reduced seed set in the form of empty spaces within siliques of self-fertilized flowers, segregation distortion; encodes a putative plasma membrane-bound hybrid histidine kinase, contains an insert within the third exon; expressed within the female gametophyte; defect in megagametogenesis caused by disruption of CKI1 leads to collapse and degeneration of the female gametophyte by stage FG7 of development and is fully penetrant by stage FG5; isolated from alpha population of the Arabidopsis Knockout Facility, pool # CSJ3939; kanamycin resistant; reported locus: At2g47430

N6377

Name: FILAMENTOUS FLOWER

Price: £11.00

Donor University of Utah Gary Drews

Locus FIL1

Stock type: individual line

Material type: seed

Description Floral primordia have reduced capacity to form flowers, giving rise to a variety of structures including: clusters of numerous flowerless pedicels (filament-like structures), stalked curled sepals, flowers with pedicel organs and long pedicels; altered floral organ number, organ position, organ identity and organ development during flower development; these defects become stronger at more acropetal positions; partially male sterile; intermediate allele.

N6378

Name: FILAMENTOUS FLOWER

Price: £11.00

Donor University of Utah Gary Drews

Locus FIL1

Stock type: individual line

Material type: seed

Description Floral primordia have reduced capacity to form flowers, giving rise to a variety of structures including: clusters of numerous flowerless pedicels (filament-like structures), stalked curled sepals, flowers with pedicel organs and long pedicels; altered floral organ number, organ position, organ identity and organ development during flower development; these defects become stronger at more acropetal positions; partially male sterile; weak allele.

N6379

Name: FILAMENTOUS FLOWER

Price: £11.00

Donor University of Utah Gary Drews

Locus FIL1

Stock type: individual line

Material type: seed

Description Floral primordia have reduced capacity to form flowers, giving rise to a variety of structures including: clusters of numerous flowerless pedicels (filament-like structures), stalked curled sepals, flowers with pedicel organs and long pedicels; altered floral organ number, organ position, organ identity and organ development during flower development; these defects become stronger at more acropetal positions; partially male sterile; strong allele.

N6380

Name: FILAMENTOUS FLOWER

Price: £11.00

Donor University of Utah Gary Drews

Locus FIL1

Stock type: individual line

Material type: seed

Description Floral primordia have reduced capacity to form flowers, giving rise to a variety of structures including: clusters of numerous flowerless pedicels (filament-like structures), stalked curled sepals, flowers with pedicel organs and long pedicels; altered floral organ number, organ position, organ identity and organ development during flower development; these defects become stronger at more acropetal positions; partially male sterile; strong allele.

N6381

Name: FILAMENTOUS FLOWER

Price: £11.00

Donor University of Utah Gary Drews

Locus FIL1

Stock type: individual line

Material type: seed

Description Floral primordia have reduced capacity to form flowers, giving rise to a variety of structures including: clusters of numerous flowerless pedicels (filament-like structures), stalked curled sepals, flowers with pedicel organs and long pedicels; altered floral organ number, organ position, organ identity and organ development during flower development; these defects become stronger at more acropetal positions; partially male sterile; strong allele.

N6394

Name: filamentous flower

Price: £11.00

Donor Kyoto University Kiyotaka Okada

Locus FIL1

Stock type: individual line

Material type: seed

Description Floral primordia have reduced capacity to form flowers, giving rise to a variety of structures including: clusters of numerous filament-like structures (underdeveloped flowers that failed to form receptacles and floral organs), stalked curled sepals, flowers with pedicel organs and long pedicels; altered floral organ number, organ position, organ identity and organ development during flower development; these defects become stronger at more acropetal positions; partially male sterile; strong allele.

N6546

Name: spiral 1

Price: £11.00

Donor Nara Institute of Science and Technology (NAIST) Takashi Hashimoto

Locus

Stock type: individual line

Material type: seed

Description Defective in directional cell elongation processes; abnormal cortical microtubule function; exhibits right-handed helical growth in roots and etiolated hypocotyls; epidermal cell files of roots are twisted to form right-handed helices; on vertically oriented hard agar plates, roots grow to the right when viewed from above the agar plates; this skewed root growth is driven by the friction between agar surface and helical epidermal cell files; phenotype is enhanced under the conditions that accelerate cell elongation, under such conditions, epidermal cells undergo isotropic cell expansion, resulting in spherically shaped cells protruding from the organ surface.

N6547

Name: spiral 1

Price: £11.00

Donor Nara Institute of Science and Technology (NAIST) Takashi Hashimoto

Locus

Stock type: individual line

Material type: seed

Description Defective in directional cell elongation processes; abnormal cortical microtubule function; exhibits right-handed helical growth in roots and etiolated hypocotyls; epidermal cell files of roots are twisted to form right-handed helices; on vertically oriented hard agar plates, roots grow to the right when viewed from above the agar plates; this skewed root growth is driven by the friction between agar surface and helical epidermal cell files; phenotype is enhanced under the conditions that accelerate cell elongation, under such conditions, epidermal cells undergo isotropic cell expansion, resulting in spherically shaped cells protruding from the organ surface.

N6586

Name: SPOROCYTELESS

Price: £11.00

Donor University of California, Davis Venkatesan Sundaresan University of California, Davis Patrick Hogan University of California, Davis Hee-Ju Yu

Locus spl (SPOROCYTELESS) or NOZZLE

Stock type: individual line

Material type: seed

Description Defective in sporocyte formation (resulting in the absence of microspores and megaspores) and anther wall formation; hypodermal cells of anthers at stage 2 enlarge and differentiate into archesporial cells normally; archesporial cells undergo periclinal divisions to form the primary parietal cell (PPC) layer and the primary sporogenous cell (PSC) layer at stage 3 anthers; PSCs do not form microsporocytes, becoming vacuolated at stage 4 anthers; parietal cells are also affected, because the PPCs go through only one division to give rise to two SPC layers that fail to divide further and differentiate; there is no endothecium or tapetum formation; anthers are composed of highly vacuolated parenchyma cells at anthesis; archesporial cells fail to elongate and differentiate into megasporocytes; nucellar development but not integument development is affected. Delayed plant senescence; sterile plants (complete male and female sterility); flowers have normal external morphology and the same number of organs as wild type, anthers are white with no pollen grains at anthesis during stage 13-14; carpels look morphologically normal but are infertile when pollinated with wild-type pollen. Identified in a screen of DS insertion lines in Arabidopsis; kanamycin resistant (50 ug/ml).

N8017

Name: Eceriferum

Price: £11.00

Donor Wageningen University Maarten Koornneef

Locus cer7

Photo

Stock type: individual line

Material type: seed

Description Form

Phenotype Bright green stem and sliques due to reduced wax layer. Semi sterile. Displays reduced plant height but plates and rods of epidermal wax are rare.

N8062

Name: pinoid

Price: £11.00

Donor Monash University David Smyth

Locus pid

Photo

Stock type: individual line

Material type: seed

Description Form, flowering

Phenotype strong allele; cotyledons often increased in number and defective in size and shape; apical meristem usually produces limited number of flowers before termination with a short pin-like stem; flowers are bunched together on the stem; no defect on polar auxin transport; floral organs are abnormal in structure, size, number and spacing within whorls; average number of sepals and stamens per flower is reduced while the number of petals is increased, stem-like gynoecium; plant height markedly reduced; maintained as heterozygote.

N8063

Name: pinoid

Price: £11.00

Donor Monash University David Smyth

Locus pid

Photo

Stock type: individual line

Material type: seed

Description Form, flowering

Phenotype intermediate allele; apical meristem produces limited number of flowers before termination of inflorescence with a short, pin-like stem; no defect in polar auxin transport; flowers abnormal (increased number of petals, reduced number of stamens, stem-like gynoecium); cotyledons often increased in number, backcrossed once. homozygous

N8064

Name: pinoid

Price: £11.00

Donor Monash University David Smyth

Locus pid

Photo

Stock type: individual line

Material type: seed

Description Flowering, Form

Phenotype strong allele; cotyledons often increased in number and defective in size and shape; apical meristem usually produces limited number of flowers before termination with a long pin-like stem; stem elongates between individual flowers; no defect on polar auxin transport; floral organs are abnormal in structure, size, number and spacing within whorls; average number of sepals and stamens per flower is reduced while the number of petals is increased, stem-like gynoecium; maintained as heterozygote; backcrossed once.

N8562

Name: cell wall mutant

Price: £11.00

Donor University of Connecticut Wolf-Dieter Reiter Purdue University Clint Chapple The Carnegie Institution of Washington Chris Somerville

Locus mur1

Stock type: individual line

Material type: seed

Description Form

Phenotype fucose content in leaf polysaccharides reduced to approximately 1 percent of wild type; slightly dwarfed; decreased tensile strength in elongating inflorescence stems; encodes GDP-mannose-4,6-dehydratase, first enzymatic step in conversion of GDP-mannose to GDP-L-fucose; same as fucose synthesis.

N8566

Name: cell wall mutant

Price: £11.00

Donor University of Connecticut Wolf-Dieter Reiter Purdue University Clint Chapple The Carnegie Institution of Washington Chris Somerville

Locus mur3

Stock type: individual line

Material type: seed

Description Form

Phenotype fucose content in leaf polysaccharides reduced to approximately 50 percent of wild type; no visible phenotypic effect.

N8571

Name: cell wall mutant

Price: £11.00

Donor University of Connecticut Wolf-Dieter Reiter Purdue University Clint Chapple The Carnegie Institution of Washington Chris Somerville

Locus mur4

Stock type: individual line

Material type: seed

Description Form

Phenotype arabinose content in leaf polysaccharides reduced to approximately 50 percent of wild type; no visible phenotypic effect.

N8576

Name: cell wall mutant

Price: £11.00

Donor University of Connecticut Wolf-Dieter Reiter Purdue University Clint Chapple The Carnegie Institution of Washington Chris Somerville

Locus mur9

Stock type: individual line

Material type: seed

Description Form

Phenotype reduced amounts of xylose and fucose in cell wall material; very slow growth; chlorotic appearance.

N8578

Name: cell wall mutant

Price: £11.00

Donor University of Connecticut Wolf-Dieter Reiter Purdue University Clint Chapple The Carnegie Institution of Washington Chris Somerville

Locus mur10

Stock type: individual line

Material type: seed

Description Form

Phenotype increased amount of arabinose in cell wall material; plants grow more slowly than wild type, and are dark-green in appearance; large reduction in fertility.

N8843

Name: auxin resistant, ethylene insensitive

Price: £11.00

Donor Adelphi University Lawrence Hobbie

Locus AUX1 EIN2

Stock type: individual line

Material type: seed

Description hormone, form, flowering

Phenotype double mutant: aux1-7, ein2. ; resistant to auxin and ethylene; large rosette, delayed bolting; altered geotropic response; slight increase in root elongation; independently isolated allele of the EIN2 locus

N8844

Name: ein2

Price: £11.00

Donor Adelphi University Lawrence Hobbie

Locus

Stock type: individual line

Material type: seed

Description independently isolated allele of the EIN2 locus; backcrossed 5 times

Phenotype insensitive to ethylene; large rosette, delayed in bolting

N16288

Name: mARF10-A

Price: £11.00

Donor Oregon State University Hiro Nonogaki Donald Danforth Plant Science Center Jim Carrington

Stock type: individual line

Material type: seed

Description ARF10:mARF10-GFP line A, transgenic line expressing a microRNA-resistant transcription factor, where the miR160-resistant form of the auxin response factor 10 (ARF10) has silent mutations in the miRNA target site (termed mARF10); the mARF10 gene (At2g28350), including the coding region (2.3 kb) with four mutations which are introduced into the microRNA160 target site and the 5'upstream region (3 kb) is fused to the green fluorescent protein; resistant to Hygromycin B (50g/ml). The genotype of this line is maintained as heterozygote; distributed seeds are the progeny of heterozygote plants, which contains homozygote mutant seeds as well as heterozygote and wild GERMPLASM_TYPE individuals. Use CS16285, CS16286 or CS16287 as control transgenic lines expressing non-resistant gene. Heterozygous for the transgene.

Phenotype Plants exhibit developmental defects in leaves, flowers and siliques. Seeds harvested from heterozygous plants produce seedlings with a range of rosette phenotypes, including: a) normal rosettes, b) relatively small rosettes with serrated leaves (32% smaller than wild-type seedlings, are able to produce viable seeds, are dominant and heterozygous), and c) extremely small rosettes with narrow leaves and lethal (cease to grow in the juvenile stage and die); the serrated- and narrow-leaf mutant phenotypes are associated with the transgene insert, whereas the normal plants lack a transgene. Plants display yellowish cauline leaves and sepals. Flowers have contorted and elongated petals and produce twisted siliques. The silique wall is wavy, such that the shape of individual seeds is apparent from the outside of the silique. During sensu stricto germination and post-germination, seeds and plants are hypersensitive to ABA in a dose-dependent manner. Mutants exhibit impaired seedling establishment (bent cotyledons, three cotyledons and three true leaves emerging at one time); although cotyledon development is altered in the mutant seedlings, the percentage seedling establishment on agar medium did not differ between wild-type and mARF10 plants during the early stages of development. The developmental defects in seedlings are more drastic when they are directly grown on soil, the smaller seedlings that eventually exhibit serrated leaves grow slowly, while most of the extremely small seedlings do not survive. Slower growth of the young mARF10 seedlings explains the reduction in size observed in plants at later stages in development.

N16289

Name: mARF10-B

Price: £11.00

Donor Oregon State University Hiro Nonogaki Donald Danforth Plant Science Center Jim Carrington

Stock type: individual line

Material type: seed

Description ARF10:mARF10-GFP line B, transgenic line expressing a microRNA-resistant transcription factor, where the miR160-resistant form of the auxin response factor 10 (ARF10) has silent mutations in the miRNA target site (termed mARF10); the mARF10 gene (At2g28350), including the coding region (2.3 kb) with four mutations which are introduced into the microRNA160 target site and the 5'upstream region (3 kb) is fused to the green fluorescent protein; resistant to Hygromycin B (50g/ml). The genotype of this line is maintained as heterozygote; distributed seeds are the progeny of heterozygote plants, which contains homozygote mutant seeds as well as heterozygote and wild GERMPLASM_TYPE individuals. Use CS16285, CS16286 or CS16287 as control transgenic lines expressing non-resistant gene. Heterozygous for the transgene.

Phenotype Plants exhibit developmental defects in leaves, flowers and siliques. Seeds harvested from heterozygous plants produce seedlings with a range of rosette phenotypes, including: a) normal rosettes, b) relatively small rosettes with serrated leaves (32% smaller than wild-type seedlings, are able to produce viable seeds, are dominant and heterozygous), and c) extremely small rosettes with narrow leaves and lethal (cease to grow in the juvenile stage and die); the serrated- and narrow-leaf mutant phenotypes are associated with the transgene insert, whereas the normal plants lack a transgene. Plants display yellowish cauline leaves and sepals. Flowers have contorted and elongated petals and produce twisted siliques. The silique wall is wavy, such that the shape of individual seeds is apparent from the outside of the silique. During sensu stricto germination and post-germination, seeds and plants are hypersensitive to ABA in a dose-dependent manner. Mutants exhibit impaired seedling establishment (bent cotyledons, three cotyledons and three true leaves emerging at one time); although cotyledon development is altered in the mutant seedlings, the percentage seedling establishment on agar medium did not differ between wild-type and mARF10 plants during the early stages of development. The developmental defects in seedlings are more drastic when they are directly grown on soil, the smaller seedlings that eventually exhibit serrated leaves grow slowly, while most of the extremely small seedlings do not survive. Slower growth of the young mARF10 seedlings explains the reduction in size observed in plants at later stages in development.

N16290

Name: mARF10-C

Price: £11.00

Donor Oregon State University Jim Carrington Oregon State University Hiro Nonogaki

Stock type: individual line

Material type: seed

Description ARF10:mARF10-GFP line C, transgenic line expressing a microRNA-resistant transcription factor, where the miR160-resistant form of the auxin response factor 10 (ARF10) has silent mutations in the miRNA target site (termed mARF10); the mARF10 gene (At2g28350), including the coding region (2.3 kb) with four mutations which are introduced into the microRNA160 target site and the 5'upstream region (3 kb) is fused to the green fluorescent protein; resistant to Hygromycin B (50æg/ml). The genotype of this line is maintained as heterozygote; distributed seeds are the progeny of heterozygote plants, which contains homozygote mutant seeds as well as heterozygote and wild type individuals. Use CS16285, CS16286 or CS16287 as control transgenic lines expressing non-resistant gene.

Phenotype Plants exhibit developmental defects in leaves, flowers and siliques. Seeds harvested from heterozygous plants produce seedlings with a range of rosette phenotypes, including: a) normal rosettes, b) relatively small rosettes with serrated leaves (32% smaller than wild-type seedlings, are able to produce viable seeds, are dominant and heterozygous), and c) extremely small rosettes with narrow leaves and lethal (cease to grow in the juvenile stage and die); the serrated- and narrow-leaf mutant phenotypes are associated with the transgene insert, whereas the normal plants lacks a transgene. Plants display yellowish cauline leaves and sepals. Flowers have contorted and elongated petals and produce twisted siliques. The silique wall is wavy, such that the shape of individual seeds is apparent from the outside of the silique. During sensu stricto germination and post-germination, seeds and plants are hypersensitive to ABA in a dose-dependent manner. Mutants exhibit impaired seedling establishment (bent cotyledons, three cotyledons and three true leaves emerging at one time); although cotyledon development is altered in the mutant seedlings, the percentage seedling establishment on agar medium did not differ between wild-type and mARF10 plants during the early stages of development. The developmental defects in seedlings are more drastic when they are directly grown on soil, the smaller seedlings that eventually exhibit serrated leaves grow slowly, while most of the extremely small seedlings do not survive. Slower growth of the young mARF10 seedlings explains the reduction in size observed in plants at later stages in development.

N16366

Name: cgl1-2

Price: £11.00

Donor Westfälische Wilhelms-Universität Münster Antje Von Schaewen

Locus cgl1

Stock type: individual line

Material type: seed

Description Originally identified in a screen of EMS-mutagenized M2 seedlings. Lacks N-acetyl-glucosaminyltransferase-I activity and is unable to form complex N-glycans in the Golgi apparatus; this point mutation affects the 3'-splice site of intron 14, resulting in frame shifts; fails to react with complex glycan antiserum on immunoblots (non-stainer).

Phenotype Longer root hairs compared to wild type.

N16367

Name: SALK_073650 homozygous

Price: £11.00

Donor Westfälische Wilhelms-Universität Münster Antje Von Schaewen

Locus cgl1

Stock type: individual line

Material type: seed

Description Confirmed line isolated from original SALK_073650 line; homozygous for the insertion; genomic PCR analyses confirmed that a single T-DNA resides within intron 11; sequence analyses of both border fragments located the T-DNA insertion at position 1792 without any base deletion; intron 11 fails to splice due to insertion of a T-DNA copy; lacks N-acetyl-glucosaminyltransferase-I activity and is unable to form complex N-glycans in the Golgi apparatus; fails to react with complex glycan antiserum on immunoblots ("non-stainer" ).

Phenotype Longer root hairs compared to wild type. Phenotype curated by ABRC

N16374

Name: 35S:VP16-IAA17mImII

Price: £11.00

Donor University of Missouri Tom Guilfoyle

Stock type: individual line

Material type: seed

Description Transgenic line expressing a stabilized form of a VP16-IAA17 activator of auxin-responsive reporter gene, expression of the modified IAA17 protein was driven by the 35S cauliflower mosaic virus promoter.

Phenotype Seedlings have long hypocotyls, long petioles, small epinastic cotyledons and leaves, short and highly branched roots, and dense root hairs. Adult plants have narrow and twisted leaves, apically dominant inflorescences, zigzag pattern of short siliques on the inflorescence stalks, and poor fertility. In several ways, the seedling and adult phenotypes resemble mutants that overproduce auxin.

N16375

Name: 35S:HA-IAA17mImII

Price: £11.00

Donor University of Missouri Tom Guilfoyle

Stock type: individual line

Material type: seed

Description Transgenic line expressing a stabilized form of an HA- IAA17 repressor of auxin-responsive reporter gene, expression of the modified IAA17 protein was driven by the 35S cauliflower mosaic virus promoter.

Phenotype Seedlings have short hypocotyls, curled down cotyledons, short petioles and unbranched primary roots. Adult plants are dwarf and bushy with short inflorescence stalks and short, wrinkled siliques. In several ways, the seedling and adult phenotypes resemble mutants with decreased auxin levels.

N16654

Name: YNACS1YCACS1

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16655

Name: YNACS2YCACS1

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16656

Name: YNACS2YCACS2

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16657

Name: YNACS4YCACS1

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16658

Name: YNACS4YCACS2

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16659

Name: YNACS4YCACS4

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16660

Name: YNACS5YCACS1

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16661

Name: YNACS5YCACS2

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16662

Name: YNACS5YCACS4

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16663

Name: YNACS5YCACS5

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16664

Name: YNACS6YCACS1

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16665

Name: YNACS6YCACS2

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16666

Name: YNACS6YCACS4

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16667

Name: YNACS6YCACS5

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16668

Name: YNACS6YCACS6

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16669

Name: YNACS7YCACS1

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16670

Name: YNACS7YCACS2

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16671

Name: YNACS7YCACS4

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16672

Name: YNACS7YCACS5

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16673

Name: YNACS7YCACS6

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.