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Search result for 'form '. Viewing records 301 to 350 of 519 hits.



N16674 Name: YNACS7YCACS7 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N16675 Name: YNACS8YCACS1 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N16676 Name: YNACS8YCACS2 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N16677 Name: YNACS8YCACS4 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N16678 Name: YNACS8YCACS5 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N16679 Name: YNACS8YCACS6 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N16680 Name: YNACS8YCACS7 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N16681 Name: YNACS8YCACS8 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N16682 Name: YNACS9YCACS1 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N16683 Name: YNACS9YCACS2 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N16684 Name: YNACS9YCACS4 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N16685 Name: YNACS9YCACS5 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N16686 Name: YNACS9YCACS6 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N16687 Name: YNACS9YCACS7 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N16688 Name: YNACS9YCACS8 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N16689 Name: YNACS9YCACS9 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N16690 Name: YNACS11YCACS1 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N16691 Name: YNACS11YCACS4 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N16692 Name: YNACS11YCACS5 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N16693 Name: YNACS11YCACS6 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N16694 Name: YNACS11YCACS7 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N16695 Name: YNACS11YCACS8 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N16696 Name: YNACS11YCACS9 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N16697 Name: YNACS11YCACS11 Price: £10.00
Donor
  • University of California, Berkeley Athanasios Theologis
Stock type: individual line
Material type: seed


Description
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
N25248 Name: deformed root hairs 3 Price: £10.00
Donor
  • University of Zurich Christoph Ringli
Locus Stock type: individual line
Material type: seed


Description
Impaired root hair development; seedlings form root hairs in a regular pattern; length of the hair proper is not affected, but side branches develop from the enlarged bases; backcrossed 3 times to C24.
N25249 Name: deformed root hairs 4 Price: £10.00
Donor
  • University of Zurich Christoph Ringli
Locus Stock type: individual line
Material type: seed


Description
Impaired root hair development; seedlings form root hairs in a regular pattern, but significantly shorter than in the wild type; root hair appears normal beside the reduced length; backcrossed 3 times to C24.
N25252 Name: deformed root hairs 7 Price: £10.00
Donor
  • University of Zurich Christoph Ringli
Locus Stock type: individual line
Material type: seed


Description
Impaired root hair development; seedlings form root hairs in a regular pattern, but significantly shorter than in the wild type; develops two different types of root hair structures: relatively short stumps with a clearly wider diameter than wild type hairs and longer hair structures which are wider in the lower half of the root hair proper while the upper half is wild type like; backcrossed 3 times to C24.
N30916 Name: NFB1 Price: £10.00
Donor
  • University of Arizona Natalie Doetsch
Locus Stock type: individual line
Material type: seed


Description
homologous to human CAF1 subunit B or yeast CAC2, the p60 subunit s of human and yeast chromatin assembly factor I , respectively. Human CAF1 is composed of three subunits that are 150 kd, 60 kd and 48 kd in size. The smallest subunit dissociates during G1 phase of the cell cycle. CAF1 is thought to deposit histones H3 and H4 onto newly replicated DNA such that histone H2A and H2B can then bind to form nucleosome core particles.
Phenotype
homologous to human CAF1 subunit B or yeast CAC2, the p60 subunit s of human and yeast chromatin assembly factor I , respectively. Human CAF1 is composed of three subunits that are 150 kd, 60 kd and 48 kd in size. The smallest subunit dissociates during G1 phase of the cell cycle. CAF1 is thought to deposit histones H3 and H4 onto newly replicated DNA such that histone H2A and H2B can then bind to form nucleosome core particles.
N30917 Name: NFB1 Price: £10.00
Donor
  • University of Arizona Natalie Doetsch
Locus Stock type: individual line
Material type: seed


Description
homologous to human CAF1 subunit B or yeast CAC2, the p60 subunit s of human and yeast chromatin assembly factor I , respectively. Human CAF1 is composed of three subunits that are 150 kd, 60 kd and 48 kd in size. The smallest subunit dissociates during G1 phase of the cell cycle. CAF1 is thought to deposit histones H3 and H4 onto newly replicated DNA such that histone H2A and H2B can then bind to form nucleosome core particles.
Phenotype
homologous to human CAF1 subunit B or yeast CAC2, the p60 subunit s of human and yeast chromatin assembly factor I , respectively. Human CAF1 is composed of three subunits that are 150 kd, 60 kd and 48 kd in size. The smallest subunit dissociates during G1 phase of the cell cycle. CAF1 is thought to deposit histones H3 and H4 onto newly replicated DNA such that histone H2A and H2B can then bind to form nucleosome core particles.
N30918 Name: NFB1 Price: £10.00
Donor
  • University of Arizona Natalie Doetsch
Locus Stock type: individual line
Material type: seed


Description
homologous to human CAF1 subunit B or yeast CAC2, the p60 subunit s of human and yeast chromatin assembly factor I , respectively. Human CAF1 is composed of three subunits that are 150 kd, 60 kd and 48 kd in size. The smallest subunit dissociates during G1 phase of the cell cycle. CAF1 is thought to deposit histones H3 and H4 onto newly replicated DNA such that histone H2A and H2B can then bind to form nucleosome core particles.
Phenotype
homologous to human CAF1 subunit B or yeast CAC2, the p60 subunit s of human and yeast chromatin assembly factor I , respectively. Human CAF1 is composed of three subunits that are 150 kd, 60 kd and 48 kd in size. The smallest subunit dissociates during G1 phase of the cell cycle. CAF1 is thought to deposit histones H3 and H4 onto newly replicated DNA such that histone H2A and H2B can then bind to form nucleosome core particles.
N30919 Name: NFB1 Price: £10.00
Donor
  • University of Arizona Natalie Doetsch
Locus Stock type: individual line
Material type: seed


Description
homologous to human CAF1 subunit B or yeast CAC2, the p60 subunit s of human and yeast chromatin assembly factor I , respectively. Human CAF1 is composed of three subunits that are 150 kd, 60 kd and 48 kd in size. The smallest subunit dissociates during G1 phase of the cell cycle. CAF1 is thought to deposit histones H3 and H4 onto newly replicated DNA such that histone H2A and H2B can then bind to form nucleosome core particles.
Phenotype
homologous to human CAF1 subunit B or yeast CAC2, the p60 subunit s of human and yeast chromatin assembly factor I , respectively. Human CAF1 is composed of three subunits that are 150 kd, 60 kd and 48 kd in size. The smallest subunit dissociates during G1 phase of the cell cycle. CAF1 is thought to deposit histones H3 and H4 onto newly replicated DNA such that histone H2A and H2B can then bind to form nucleosome core particles.
N42994 Name: SLAT Filter Type 2 - NASC style Price: £15.00
Donor
  • University of Nottingham Sean May
Stock type: individual line
Material type: seed


Description
filter.Filter type 2: NASC style filter representing 60,000 lines (WITH MARKER); uses the original Sainsbury laboratory DNAs, and the original SL IPCR products; rearrayed in a linear form for ease of picking pools.
N69096 Name: p35S:HF-GFP-RPL18 Price: £10.00
Donor
  • University of California, Riverside Julia Bailey-Serres
Stock type: individual line
Material type: seed


Description
The 35S promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, sGFP, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-sGFP-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).
Phenotype
No visible phenotype.
N69097 Name: pCER5:HF-GFP-RPL18 Price: £10.00
Donor
  • University of California, Riverside Julia Bailey-Serres
Stock type: individual line
Material type: seed


Description
The CER5 promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, sGFP, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-sGFP-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).
Phenotype
No visible phenotype.
N69098 Name: pCO2:HF-GFP-RPL18 Price: £10.00
Donor
  • University of California, Riverside Julia Bailey-Serres
Stock type: individual line
Material type: seed


Description
The CO2 promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, sGFP, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-sGFP-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).
Phenotype
No visible phenotype.
N69099 Name: pKAT1:HF-GFP-RPL18 Price: £10.00
Donor
  • University of California, Riverside Julia Bailey-Serres
Stock type: individual line
Material type: seed


Description
The KAT1 promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, sGFP, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-sGFP-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).
Phenotype
No visible phenotype.
N69100 Name: pLAT52:HF-RPL18 Price: £10.00
Donor
  • University of California, Riverside Julia Bailey-Serres
Stock type: individual line
Material type: seed


Description
The Solanum lycopersicum LAT52 promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).
Phenotype
No visible phenotype.
N69101 Name: pPEP:HF-GFP-RPL18 Price: £10.00
Donor
  • University of California, Riverside Julia Bailey-Serres
Stock type: individual line
Material type: seed


Description
The PEP promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, sGFP, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-sGFP-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).
Phenotype
No visible phenotype.
N69102 Name: pRBCS:HF-GFP-RPL18 Price: £10.00
Donor
  • University of California, Riverside Julia Bailey-Serres
Stock type: individual line
Material type: seed


Description
The RBSC promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, sGFP, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-sGFP-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).
Phenotype
No visible phenotype.
N69103 Name: pRPL11C:HF-RPL18 Price: £10.00
Donor
  • University of California, Riverside Julia Bailey-Serres
Stock type: individual line
Material type: seed


Description
The RPL11C promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).
Phenotype
No visible phenotype.
N69104 Name: pRPL11C:HF-GFP-RPL18 Price: £10.00
Donor
  • University of California, Riverside Julia Bailey-Serres
Stock type: individual line
Material type: seed


Description
The RPL11C promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, sGFP, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-sGFP-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).
Phenotype
No visible phenotype.
N69105 Name: pSCR:HF-GFP-RPL18 Price: £10.00
Donor
  • University of California, Riverside Julia Bailey-Serres
Stock type: individual line
Material type: seed


Description
The SCR promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, sGFP, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-sGFP-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).
Phenotype
No visible phenotype.
N69106 Name: pSUC2:HF-GFP-RPL18 Price: £10.00
Donor
  • University of California, Riverside Julia Bailey-Serres
Stock type: individual line
Material type: seed


Description
The SUC2 promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, sGFP, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-sGFP-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).
Phenotype
No visible phenotype.
N69107 Name: pSULTR2;1:HF-RPL18 Price: £10.00
Donor
  • University of California, Riverside Julia Bailey-Serres
Stock type: individual line
Material type: seed


Description
The SULTR2;1 promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).
Phenotype
No visible phenotype.
N69108 Name: pWOL:HF-GFP-RPL18 Price: £10.00
Donor
  • University of California, Riverside Julia Bailey-Serres
Stock type: individual line
Material type: seed


Description
The WOL promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, sGFP, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-sGFP-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).
Phenotype
No visible phenotype.
N69886 Name: 69886 Price: £10.00
Donor
  • Leiden University Bert J. van der Zaal
Locus Stock type: individual line
Material type: seed


Description
Phosphomimetic form of RACK1A, RACK1A (S122D/T162E), driven by the native promoter of RACK1A in pGWB40 was transformed into the rack1a-2 (CS68987) mutant background. Kanamycin and Hygromycin resistant. Genotype of transgene is unknown.
Phenotype
Hypersensitivity to glucose. Reduced number of rosette leaves, late flowering.
N69887 Name: 69887 Price: £10.00
Donor
  • Leiden University Bert J. van der Zaal
Locus Stock type: individual line
Material type: seed


Description
Phosphomimetic form of RACK1A, RACK1A (S122D/T162E), driven by the native promoter of RACK1A in pGWB40 was transformed into the rack1a-2 (CS68987) mutant background. Kanamycin and Hygromycin resistant. Genotype of transgene is unknown.
Phenotype
Hypersensitivity to glucose. Reduced number of rosette leaves, late flowering.
N69888 Name: 69888 Price: £10.00
Donor
  • Leiden University Bert J. van der Zaal
Locus Stock type: individual line
Material type: seed


Description
Phosphorylation-dead form of RACK1A, RACK1A (S122A/T162A), driven by the native promoter of RACK1A in pGWB40 was transformed into the rack1a-2 (CS68987) mutant background. Kanamycin and Hygromycin resistant. Genotype of transgene is unknown.
Phenotype
No visible phenotype
N69889 Name: 69889 Price: £10.00
Donor
  • Leiden University Bert J. van der Zaal
Locus Stock type: individual line
Material type: seed


Description
Phosphorylation-dead form of RACK1A, RACK1A (S122A/T162A), driven by the native promoter of RACK1A in pGWB40 was transformed into the rack1a-2 (CS68987) mutant background. Kanamycin and Hygromycin resistant. Genotype of transgene is unknown.
Phenotype
No visible phenotype
N70001 Name: 1 Price: £10.00
Donor
  • University of Pennsylvania Scott Poethig
Stock type: individual line
Material type: seed


Description
line transformed with the enhancer trap vector GAL4-VP16 under the regulation of a minimal CaMV 35S promoter; expression of this vector is monitored by the reporter gene UAS::mGFP5-ER, an endoplasmic reticulum ER-localized form of the GFP gene; no visible phenotype; kanamycin resistant; T4 generation.
Phenotype
no visible phenotype