NASC

N16674

Name: YNACS7YCACS7

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16675

Name: YNACS8YCACS1

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16676

Name: YNACS8YCACS2

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16677

Name: YNACS8YCACS4

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16678

Name: YNACS8YCACS5

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16679

Name: YNACS8YCACS6

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16680

Name: YNACS8YCACS7

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16681

Name: YNACS8YCACS8

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16682

Name: YNACS9YCACS1

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16683

Name: YNACS9YCACS2

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16684

Name: YNACS9YCACS4

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16685

Name: YNACS9YCACS5

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16686

Name: YNACS9YCACS6

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16687

Name: YNACS9YCACS7

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16688

Name: YNACS9YCACS8

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16689

Name: YNACS9YCACS9

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16690

Name: YNACS11YCACS1

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16691

Name: YNACS11YCACS4

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16692

Name: YNACS11YCACS5

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16693

Name: YNACS11YCACS6

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16694

Name: YNACS11YCACS7

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16695

Name: YNACS11YCACS8

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16696

Name: YNACS11YCACS9

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N16697

Name: YNACS11YCACS11

Price: £11.00

Donor University of California, Berkeley Athanasios Theologis

Stock type: individual line

Material type: seed

Description YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.

N25248

Name: deformed root hairs 3

Price: £11.00

Donor University of Zurich Christoph Ringli

Locus der3

Stock type: individual line

Material type: seed

Description Impaired root hair development; seedlings form root hairs in a regular pattern; length of the hair proper is not affected, but side branches develop from the enlarged bases; backcrossed 3 times to C24.

N25249

Name: deformed root hairs 4

Price: £11.00

Donor University of Zurich Christoph Ringli

Locus der4

Stock type: individual line

Material type: seed

Description Impaired root hair development; seedlings form root hairs in a regular pattern, but significantly shorter than in the wild type; root hair appears normal beside the reduced length; backcrossed 3 times to C24.

N25252

Name: deformed root hairs 7

Price: £11.00

Donor University of Zurich Christoph Ringli

Locus der7

Stock type: individual line

Material type: seed

Description Impaired root hair development; seedlings form root hairs in a regular pattern, but significantly shorter than in the wild type; develops two different types of root hair structures: relatively short stumps with a clearly wider diameter than wild type hairs and longer hair structures which are wider in the lower half of the root hair proper while the upper half is wild type like; backcrossed 3 times to C24.

N30916

Name: NFB1

Price: £11.00

Donor University of Arizona Natalie Doetsch

Locus NFB1

Stock type: individual line

Material type: seed

Description homologous to human CAF1 subunit B or yeast CAC2, the p60 subunit s of human and yeast chromatin assembly factor I , respectively. Human CAF1 is composed of three subunits that are 150 kd, 60 kd and 48 kd in size. The smallest subunit dissociates during G1 phase of the cell cycle. CAF1 is thought to deposit histones H3 and H4 onto newly replicated DNA such that histone H2A and H2B can then bind to form nucleosome core particles.

Phenotype homologous to human CAF1 subunit B or yeast CAC2, the p60 subunit s of human and yeast chromatin assembly factor I , respectively. Human CAF1 is composed of three subunits that are 150 kd, 60 kd and 48 kd in size. The smallest subunit dissociates during G1 phase of the cell cycle. CAF1 is thought to deposit histones H3 and H4 onto newly replicated DNA such that histone H2A and H2B can then bind to form nucleosome core particles.

N30917

Name: NFB1

Price: £11.00

Donor University of Arizona Natalie Doetsch

Locus NFB1

Stock type: individual line

Material type: seed

Description homologous to human CAF1 subunit B or yeast CAC2, the p60 subunit s of human and yeast chromatin assembly factor I , respectively. Human CAF1 is composed of three subunits that are 150 kd, 60 kd and 48 kd in size. The smallest subunit dissociates during G1 phase of the cell cycle. CAF1 is thought to deposit histones H3 and H4 onto newly replicated DNA such that histone H2A and H2B can then bind to form nucleosome core particles.

Phenotype homologous to human CAF1 subunit B or yeast CAC2, the p60 subunit s of human and yeast chromatin assembly factor I , respectively. Human CAF1 is composed of three subunits that are 150 kd, 60 kd and 48 kd in size. The smallest subunit dissociates during G1 phase of the cell cycle. CAF1 is thought to deposit histones H3 and H4 onto newly replicated DNA such that histone H2A and H2B can then bind to form nucleosome core particles.

N30918

Name: NFB1

Price: £11.00

Donor University of Arizona Natalie Doetsch

Locus NFB1

Stock type: individual line

Material type: seed

Description homologous to human CAF1 subunit B or yeast CAC2, the p60 subunit s of human and yeast chromatin assembly factor I , respectively. Human CAF1 is composed of three subunits that are 150 kd, 60 kd and 48 kd in size. The smallest subunit dissociates during G1 phase of the cell cycle. CAF1 is thought to deposit histones H3 and H4 onto newly replicated DNA such that histone H2A and H2B can then bind to form nucleosome core particles.

Phenotype homologous to human CAF1 subunit B or yeast CAC2, the p60 subunit s of human and yeast chromatin assembly factor I , respectively. Human CAF1 is composed of three subunits that are 150 kd, 60 kd and 48 kd in size. The smallest subunit dissociates during G1 phase of the cell cycle. CAF1 is thought to deposit histones H3 and H4 onto newly replicated DNA such that histone H2A and H2B can then bind to form nucleosome core particles.

N30919

Name: NFB1

Price: £11.00

Donor University of Arizona Natalie Doetsch

Locus NFB1

Stock type: individual line

Material type: seed

Description homologous to human CAF1 subunit B or yeast CAC2, the p60 subunit s of human and yeast chromatin assembly factor I , respectively. Human CAF1 is composed of three subunits that are 150 kd, 60 kd and 48 kd in size. The smallest subunit dissociates during G1 phase of the cell cycle. CAF1 is thought to deposit histones H3 and H4 onto newly replicated DNA such that histone H2A and H2B can then bind to form nucleosome core particles.

Phenotype homologous to human CAF1 subunit B or yeast CAC2, the p60 subunit s of human and yeast chromatin assembly factor I , respectively. Human CAF1 is composed of three subunits that are 150 kd, 60 kd and 48 kd in size. The smallest subunit dissociates during G1 phase of the cell cycle. CAF1 is thought to deposit histones H3 and H4 onto newly replicated DNA such that histone H2A and H2B can then bind to form nucleosome core particles.

N42994

Name: SLAT Filter Type 2 - NASC style

Price: £15.00

Donor University of Nottingham Sean May

Stock type: individual line

Material type: seed

Description filter.Filter type 2: NASC style filter representing 60,000 lines (WITH MARKER); uses the original Sainsbury laboratory DNAs, and the original SL IPCR products; rearrayed in a linear form for ease of picking pools.

N69096

Name: p35S:HF-GFP-RPL18

Price: £11.00

Donor University of California, Riverside Julia Bailey-Serres

Stock type: individual line

Material type: seed

Description The 35S promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, sGFP, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-sGFP-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).

Phenotype No visible phenotype.

N69097

Name: pCER5:HF-GFP-RPL18

Price: £11.00

Donor University of California, Riverside Julia Bailey-Serres

Stock type: individual line

Material type: seed

Description The CER5 promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, sGFP, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-sGFP-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).

Phenotype No visible phenotype.

N69098

Name: pCO2:HF-GFP-RPL18

Price: £11.00

Donor University of California, Riverside Julia Bailey-Serres

Stock type: individual line

Material type: seed

Description The CO2 promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, sGFP, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-sGFP-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).

Phenotype No visible phenotype.

N69099

Name: pKAT1:HF-GFP-RPL18

Price: £11.00

Donor University of California, Riverside Julia Bailey-Serres

Stock type: individual line

Material type: seed

Description The KAT1 promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, sGFP, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-sGFP-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).

Phenotype No visible phenotype.

N69100

Name: pLAT52:HF-RPL18

Price: £11.00

Donor University of California, Riverside Julia Bailey-Serres

Stock type: individual line

Material type: seed

Description The Solanum lycopersicum LAT52 promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).

Phenotype No visible phenotype.

N69101

Name: pPEP:HF-GFP-RPL18

Price: £11.00

Donor University of California, Riverside Julia Bailey-Serres

Stock type: individual line

Material type: seed

Description The PEP promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, sGFP, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-sGFP-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).

Phenotype No visible phenotype.

N69102

Name: pRBCS:HF-GFP-RPL18

Price: £11.00

Donor University of California, Riverside Julia Bailey-Serres

Stock type: individual line

Material type: seed

Description The RBSC promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, sGFP, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-sGFP-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).

Phenotype No visible phenotype.

N69103

Name: pRPL11C:HF-RPL18

Price: £11.00

Donor University of California, Riverside Julia Bailey-Serres

Stock type: individual line

Material type: seed

Description The RPL11C promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).

Phenotype No visible phenotype.

N69104

Name: pRPL11C:HF-GFP-RPL18

Price: £11.00

Donor University of California, Riverside Julia Bailey-Serres

Stock type: individual line

Material type: seed

Description The RPL11C promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, sGFP, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-sGFP-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).

Phenotype No visible phenotype.

N69105

Name: pSCR:HF-GFP-RPL18

Price: £11.00

Donor University of California, Riverside Julia Bailey-Serres

Stock type: individual line

Material type: seed

Description The SCR promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, sGFP, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-sGFP-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).

Phenotype No visible phenotype.

N69106

Name: pSUC2:HF-GFP-RPL18

Price: £11.00

Donor University of California, Riverside Julia Bailey-Serres

Stock type: individual line

Material type: seed

Description The SUC2 promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, sGFP, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-sGFP-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).

Phenotype No visible phenotype.

N69107

Name: pSULTR2;1:HF-RPL18

Price: £11.00

Donor University of California, Riverside Julia Bailey-Serres

Stock type: individual line

Material type: seed

Description The SULTR2;1 promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).

Phenotype No visible phenotype.

N69108

Name: pWOL:HF-GFP-RPL18

Price: £11.00

Donor University of California, Riverside Julia Bailey-Serres

Stock type: individual line

Material type: seed

Description The WOL promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, sGFP, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-sGFP-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).

Phenotype No visible phenotype.

N69886

Name: 69886

Price: £11.00

Donor Leiden University Bert J. van der Zaal

Locus

Stock type: individual line

Material type: seed

Description Phosphomimetic form of RACK1A, RACK1A (S122D/T162E), driven by the native promoter of RACK1A in pGWB40 was transformed into the rack1a-2 (CS68987) mutant background. Kanamycin and Hygromycin resistant. Genotype of transgene is unknown.

Phenotype Hypersensitivity to glucose. Reduced number of rosette leaves, late flowering.

N69887

Name: 69887

Price: £11.00

Donor Leiden University Bert J. van der Zaal

Locus

Stock type: individual line

Material type: seed

Description Phosphomimetic form of RACK1A, RACK1A (S122D/T162E), driven by the native promoter of RACK1A in pGWB40 was transformed into the rack1a-2 (CS68987) mutant background. Kanamycin and Hygromycin resistant. Genotype of transgene is unknown.

Phenotype Hypersensitivity to glucose. Reduced number of rosette leaves, late flowering.

N69888

Name: 69888

Price: £11.00

Donor Leiden University Bert J. van der Zaal

Locus

Stock type: individual line

Material type: seed

Description Phosphorylation-dead form of RACK1A, RACK1A (S122A/T162A), driven by the native promoter of RACK1A in pGWB40 was transformed into the rack1a-2 (CS68987) mutant background. Kanamycin and Hygromycin resistant. Genotype of transgene is unknown.

Phenotype No visible phenotype

N69889

Name: 69889

Price: £11.00

Donor Leiden University Bert J. van der Zaal

Locus

Stock type: individual line

Material type: seed

Description Phosphorylation-dead form of RACK1A, RACK1A (S122A/T162A), driven by the native promoter of RACK1A in pGWB40 was transformed into the rack1a-2 (CS68987) mutant background. Kanamycin and Hygromycin resistant. Genotype of transgene is unknown.

Phenotype No visible phenotype

N70001

Name: 1

Price: £11.00

Donor University of Pennsylvania Scott Poethig

Stock type: individual line

Material type: seed

Description line transformed with the enhancer trap vector GAL4-VP16 under the regulation of a minimal CaMV 35S promoter; expression of this vector is monitored by the reporter gene UAS::mGFP5-ER, an endoplasmic reticulum ER-localized form of the GFP gene; no visible phenotype; kanamycin resistant; T4 generation.

Phenotype no visible phenotype