NASC Stock Detail Page
NASC ID: N101032
Name: AT_3383
ABRC stock number: CS101032
Description: Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Donation Date: 2001-06-05
| Donated by: | John Innes Centre Caroline Dean |
Donor Number: AT_3383
Part of : JIC Activate lines
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Seed type: Activate lines/ds tagged
Mutagen: transposon insertion
Background: Ler(Landsberg_erecta)(NW20)
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.