NASC Stock Detail Page
NASC ID: N16290
Name: mARF10-C
ABRC stock number: CS16290
Description: ARF10:mARF10-GFP line C, transgenic line expressing a microRNA-resistant transcription factor, where the miR160-resistant form of the auxin response factor 10 (ARF10) has silent mutations in the miRNA target site (termed mARF10); the mARF10 gene (At2g28350), including the coding region (2.3 kb) with four mutations which are introduced into the microRNA160 target site and the 5'upstream region (3 kb) is fused to the green fluorescent protein; resistant to Hygromycin B (50æg/ml). The genotype of this line is maintained as heterozygote; distributed seeds are the progeny of heterozygote plants, which contains homozygote mutant seeds as well as heterozygote and wild type individuals. Use CS16285, CS16286 or CS16287 as control transgenic lines expressing non-resistant gene.
Donation Date: 2007-12-05
| Donated by: | Oregon State University Jim Carrington Oregon State University Hiro Nonogaki |
Donor Number: mARF10-C
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Seed type: Transgenic
Background: Col(Columbia)
Growth requirement: hygromycin B resistant (50 ug/ml), maintain as heterozygote. Use CS16285, CS16286 or CS16287 as control transgenic lines expressing non-resistant gene.
Phenotype
Plants exhibit developmental defects in leaves, flowers and siliques. Seeds harvested from heterozygous plants produce seedlings with a range of rosette phenotypes, including: a) normal rosettes, b) relatively small rosettes with serrated leaves (32% smaller than wild-type seedlings, are able to produce viable seeds, are dominant and heterozygous), and c) extremely small rosettes with narrow leaves and lethal (cease to grow in the juvenile stage and die); the serrated- and narrow-leaf mutant phenotypes are associated with the transgene insert, whereas the normal plants lacks a transgene. Plants display yellowish cauline leaves and sepals. Flowers have contorted and elongated petals and produce twisted siliques. The silique wall is wavy, such that the shape of individual seeds is apparent from the outside of the silique. During sensu stricto germination and post-germination, seeds and plants are hypersensitive to ABA in a dose-dependent manner. Mutants exhibit impaired seedling establishment (bent cotyledons, three cotyledons and three true leaves emerging at one time); although cotyledon development is altered in the mutant seedlings, the percentage seedling establishment on agar medium did not differ between wild-type and mARF10 plants during the early stages of development. The developmental defects in seedlings are more drastic when they are directly grown on soil, the smaller seedlings that eventually exhibit serrated leaves grow slowly, while most of the extremely small seedlings do not survive. Slower growth of the young mARF10 seedlings explains the reduction in size observed in plants at later stages in development.