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NASC

The European Arabidopsis Stock Centre

NASC Stock Detail Page

NASC ID: N16366

Name: cgl1-2

ABRC stock number: CS16366

Description: Originally identified in a screen of EMS-mutagenized M2 seedlings. Lacks N-acetyl-glucosaminyltransferase-I activity and is unable to form complex N-glycans in the Golgi apparatus; this point mutation affects the 3'-splice site of intron 14, resulting in frame shifts; fails to react with complex glycan antiserum on immunoblots (non-stainer).

Donation Date: 2008-09-17

Donated by:  Westfälische Wilhelms-Universität Münster Antje Von Schaewen

Donor Number: C6

Stock type: individual line

Material type: seed

Status Price (£)
Available £11.00

Germplasm Info

Seed type: Mutant

Mutagen: ethylmethane sulfonate

Background: Columbia

Segregation status: homozygous

Pedigree: Backcrossed once to Ler and 7 times to Col.

Growth requirement: none

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:
  • cgl1-2
  • cgl1

Phenotype

Longer root hairs compared to wild type.

References

Frank, J. et al. 2008. Comparative analyses of Arabidopsis complex glycan1 mutants and genetic interaction with staurosporin and temperature sensitive3a. Plant physiology 148 (3):1354-67 .PubMed ID: 18768906.