NASC Stock Detail Page
NASC ID: N16696
Name: YNACS11YCACS9
ABRC stock number: CS16696
Description: YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
Donation Date: 2009-09-10
| Donated by: | University of California, Berkeley Athanasios Theologis |
Donor Number: YNACS11YCACS9
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: T-DNA Insertion
Background: Col(Columbia)
References
Tsuchisaka, A., et al. 2009. A Combinatorial Interplay Among the 1-Aminocyclopropane-1-carboxylate Isoforms Regulates Ethylene Biosynthesis in Arabidopsis thaliana. Genetics 183(3):979-1003.PubMed ID: 19752216.