NASC Stock Detail Page
NASC ID: N224
Name: AIR1-8 (transgenic reporter line in Columbia (gl1)
ABRC stock number: CS224
Donation Date: 1992-06-01
| Donated by: | Leiden University Bert J. van der Zaal |
Donor Number: AIR1-8
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: T-DNA Insertion
Background: Columbia (gl1)
Segregation status: homozygous
Marker used: Kanamycin segregation Resistant:Senstive(Kan R)
Segregation: No
Transformation method: The reporter gene construct AIR1 was introduced into the parent line AIR1-8 by means of a root cocultivation method using Agrobacterium tumefaciens (see reference for details).
Phenotype
Auxin induced kanamycin/GUS reporter line. Plants contain a T-DNA construct in which the promoter of an auxin-inducible glutathione S-transferase (GST) gene from tobacco has been fused to the kanamycin resistance gene (nptII) as well as to the b-glucuronidase (gusA) reporter gene. Used as a background for mutagenesis/selection of auxin related genes.
References
van der Kop, D.A. et al. 1999. Selection of Arabidopsis mutants overexpressing genes driven by the promoter of an auxin-inducible glutathione S-transferase gene. Plant Molecular Biology 39(5):979-90.PubMed ID: 10344203.