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NASC Stock Detail Page

NASC ID: N225

Name: glutathione up-regulated

ABRC stock number: CS225

Description: hormone, biochemical

Donation Date: 1992-06-01

Donated by:  Leiden University Bert J. van der Zaal

Stock type: individual line

Material type: seed

Status Price (£)
Available £10.00

Germplasm Info

Mutagen: ethylmethane sulfonate

Background: AIR1-8 (N224)

Pedigree: gup1-1 was backcrossed once to AIR1-8 (and homozygous F3 lines were selected).

Marker used: Kanamycin segregation Resistant:Senstive(Kan R)

Segregation: No

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:
  • gl1
  • gup1-1
  • gl1
  • GUP1

Phenotype

Both gup1-1 and gup2-1 (stocck number N226) have no clear phenotype when grown in soil. When grown in vitro, both exhibit epinastic growth of leaves. Root growth is slightly more sensitive to IAA inhibition of elongation. Both gup1-1 and gup2-1 lead to enhanced kanamycin resistance in the AIR1-8 background via an nptII gene under control of a tobacco GST promoter . gup1-1 also shows an increased mRNA level of GST5 from Arabidopsis (designated At103-1a in van der Kop et. al. 1999).

References

van der Kop, D.A. et al. 1999. Selection of Arabidopsis mutants overexpressing genes driven by the promoter of an auxin-inducible glutathione S-transferase gene. Plant Molecular Biology 39(5):979-90.PubMed ID: 10344203.