NASC Stock Detail Page
NASC ID: N225
Name: glutathione up-regulated
ABRC stock number: CS225
Description: hormone, biochemical
Donation Date: 1992-06-01
| Donated by: | Leiden University Bert J. van der Zaal |
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ethylmethane sulfonate
Background: AIR1-8 (N224)
Pedigree: gup1-1 was backcrossed once to AIR1-8 (and homozygous F3 lines were selected).
Marker used: Kanamycin segregation Resistant:Senstive(Kan R)
Segregation: No
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Both gup1-1 and gup2-1 (stocck number N226) have no clear phenotype when grown in soil. When grown in vitro, both exhibit epinastic growth of leaves. Root growth is slightly more sensitive to IAA inhibition of elongation. Both gup1-1 and gup2-1 lead to enhanced kanamycin resistance in the AIR1-8 background via an nptII gene under control of a tobacco GST promoter . gup1-1 also shows an increased mRNA level of GST5 from Arabidopsis (designated At103-1a in van der Kop et. al. 1999).
References
van der Kop, D.A. et al. 1999. Selection of Arabidopsis mutants overexpressing genes driven by the promoter of an auxin-inducible glutathione S-transferase gene. Plant Molecular Biology 39(5):979-90.PubMed ID: 10344203.