NASC Stock Detail Page
NASC ID: N23886
Name: twin (twn); twin1 (twn1); emb214
ABRC stock number: CS23886
Description: twn1 seeds were from a large collection of embryo-defective mutants identified by the Meinke laboratory (Errampalli et al., Plant Cell, 1991), from the K. Feldmann T-DNA collection. Does not contain a functional T-DNA insert, no antibiotic resistance. Submitted seeds obtained from a selfed twn1 homozygote, previously backcrossed more than once to Ws.
Donation Date: 2009-09-30
| Donated by: | Whitman College Daniel Vernon |
Donor Number: twin (twn); twin1 (twn1); emb214
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: T-DNA Insertion
Background: Ws-2(N2360)
Segregation status: homozygous
Pedigree: Submitted seeds obtained from a selfed twn1 homozygote, previously backcrossed more than once to Ws.
Growth requirement: Germinate on agar plates with MS media + 1% sucrose, to observe germination of small secondary (twin) seedlings
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Incomplete penetrance and highly variable phenotypein mutant embryos. About 8 % of seeds contain embryos that display suspensor proliferation or polyembryony; Some twin embryos are viable and germinate. In addition to twinning, some embryos show variable defects in the embryo proper early in embryogenesis. Cotyledon defects are present in about 10% of embryos/seedlings and occur independent of twinning. Defects in cotyledons/leaf organization do not extend into post-embryonic development beyond the first leaf pair. Mutant phenotype(s) appears to be embryo-specific.
References
Vernon, D.M. & Meinke, D.W. 1994. Embryogenic transformation of the suspensor in twin, a polyembryonic mutant of Arabidopsis. Developmental biology 165(2):566-73.PubMed ID: 7958422.
Vernon, D.M. et al. 2001. An expanded role for the TWN1 gene in embryogenesis: defects in cotyledon pattern and morphology in the twn1 mutant of Arabidopsis (Brassicaceae). American journal of botany 88(4):570-82.PubMed ID: 11302841