NASC Stock Detail Page
NASC ID: N29391
Name: RP217
ABRC stock number: CS29391
Description: Whole-genome sequenced inbred Boechera stricta accession; admixed population group; included in panel (CS29669) for genome-wide association studies examining flowering variation among environments and associations with climate conditions in home environments; seeds were obtained from a single mother plant and grown in the greenhouse for at least one generation
Donation Date: 2018-12-17
| Donated by: | Max-Planck-Institut für Chemische Ökologie Thomas Mitchell-Olds University of Georgia Jill T Anderson HudsonAlpha Institute for Biotechnology John T Lovell |
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available to order at NASC | £11.00 |
Germplasm Info
Genus: Boechera Species: stricta
Seed type: Ecotype
Growth requirement: For germination and establishment, seeds are stratified in Petri dishes with damp filter paper, and then moved to a dark room at 4°C for 8 days, and then placed in a greenhouse or growth chamber for 6 days (day length: 16h; night length: 8h; humidity: 70%; temperature: 25 - 30°C). Germinated seedlings are randomized into soil-filled containers. Flats or pots can be covered with clear plastic domes for 9 days to maintain humidity. Most genotypes require cold vernalization to flower. After establishment, seedlings should be watered and fertilized as needed, until rosettes reach about 6 cm in diameter. Cold vernalization entails 4°C, ~40% relative humidity, 12h day length for 6 - 8 weeks. Flowering time may depend on length of vernalization, and generation time can be minimized with approximately 8 weeks of cold.
Ecotype data
Location: WY
Country: USA
Altitude Min: 2851
Altitude Max: 2851
Latitude Min: 43.003
Latitude Max: 43.003
Longitude Min: -109.754
Longitude Max: -109.754
References
Wenjie Yan, Baosheng Wang, Emily Chan, Thomas Mitchell-Olds; 2021-05-01; Genetic architecture and adaptation of flowering time among environments; New Phytol; PubMed ID: 33484593
Baosheng Wang, Julius P Mojica, Nadeesha Perera, Cheng-Ruei Lee, John T Lovell, Aditi Sharma, Catherine Adam, Anna Lipzen, Kerrie Barry, Daniel S Rokhsar, Jeremy Schmutz, Thomas Mitchell-Old; 2019-08-09; Correction to: Ancient polymorphisms contribute to genome-wide variation by long-term balancing selection and divergent sorting in Boechera stricta; Genome Biol.; PubMed ID: 31399118
Baosheng Wang, Julius P Mojica, Nadeesha Perera, Cheng-Ruei Lee, John T Lovell, Aditi Sharma, Catherine Adam, Anna Lipzen, Kerrie Barry, Daniel S Rokhsar, Jeremy Schmutz , Thomas Mitchell-Olds; 2019-06-21; Ancient polymorphisms contribute to genome-wide variation by long-term balancing selection and divergent sorting in Boechera stricta; Genome Biol .; PubMed ID: 31227026