NASC Stock Detail Page
NASC ID: N501847
Name: SALK_001847
ABRC stock number: salk_001847
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_001847.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G73020.1 (at chr 1 pos 27473141 (W/27473092-27473141) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 1, R 2, C 1 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO416657. SALKseq_001847.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G62980.1=P355 and AT3G62988.1 (at chr 3 pos 23281428 (W/23281351-23281428) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 1, R 2, C 1 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO419975. SALKseq_001847.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G23990.1 (at chr 3 pos 8668891 (W/8668829-8668891) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 1, R 2, C 1 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO423291. SALKseq_001847.5 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G28750.1 (at chr 5 pos 10785230 (C/10785230-10785295) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 1, R 2, C 1 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO426609.
Donation Date: 2001-01-11
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_001847
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_001847C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.